Endy:Screening plasmid: Difference between revisions
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#Brighter FPs (in particular RFP is very dim) | #Brighter FPs (in particular RFP is very dim) | ||
#Lower copy plasmid | #Lower copy plasmid | ||
==References== | |||
[2] Khlebnikov et al,Modulation of gene expression from the arabinose-inducible araBAD promoter. J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7. | |||
[3] Effect of gene location, mRNA secondary structures, and RNase sites on expression of two genes in an engineered operon. Biotechnol Bioeng. 2002 Dec 30;80(7):762-76. | |||
__NOTOC__ | __NOTOC__ |
Revision as of 19:37, 9 May 2006
This page is a work in progress.
Introduction
The screening plasmid is designed to enable fast characterization of PoPS-based parts and devices. It consists of 4 components: (1) Tunable input (2) Input measurement (3) Part/Device insertion site (4) Output measurement.
Screening Plasmid 1.0
Terminator Characterization via Screening Plasmid 1.0
Inverter Characterization via Screening Plasmid 1.0
Screening Plasmid 2.0
We are in the process of designing a new version of the screening plasmid to account for some of the shortcomings of the previous version. An early list of possible improvements:
- Brighter FPs (in particular RFP is very dim)
- Lower copy plasmid
References
[2] Khlebnikov et al,Modulation of gene expression from the arabinose-inducible araBAD promoter. J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7.
[3] Effect of gene location, mRNA secondary structures, and RNase sites on expression of two genes in an engineered operon. Biotechnol Bioeng. 2002 Dec 30;80(7):762-76.