Endy:Screening plasmid/Constructs

Version 0

• Version 0.1
  • I13512 - PBad RFP GFP Term
  • I13513 - PBad RFP Insertion GFP Term
  • I13514 - PBad GFP Insertion RFP Term

• Version 0.2
  • I13529 - RFP upstream, GFP downstream, one RNAseE site in between.
  • I13530 - GFP upstream, RFP downstream, one RNAseE site in between.
  • I13533 - One promoter. Insertion site has flanking RNAseE sites. GFP is downstream and has a 5' hairpin.
  • I13534 - One promoter. Insertion site has flanking RNAseE sites. RFP is downstream and has a 5' hairpin.

• Version 0.3
  • I13537 - Two promoters. I13458 split out, I13453 drives both GFP and RFP.
  • I13538 - Two promoters. I0500 drives GFP, I13453 drives RFP
  • I13539 - Two promoters. I0500 drives RFP, I13453 drives GFP

Version 1

• Version 1.0
  • Version 0.2 - I13534 was released as Screening Plasmid v1.0 (pSB1A10) on the MIT Registry of Biological Parts following promising results in screening/characterization of terminators and inverters.
    • Design - One promoter. Insertion site has flanking RNAseE sites. RFP is downstream and has a 5' hairpin.

Controls

• I13543 - Pbad driving RNAseE/hairpin/RFP - inducible promoter
• <bbpart>I13522</bbpart> - constitutive GFP
• <bbpart>I13521</bbpart> - constitutive RFP
• <bbpart>I13540</bbpart> - inducible GFP
• <bbpart>I13520</bbpart> - inducible RFP
• <bbpart>V1023</bbpart> - negative (CW2553/pJAt8)