Endy:Screening plasmid/Inverter characterization: Difference between revisions
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*tetR QPI | |||
[[Image:June22_2006_tetR_QPI_rep1.png]] | |||
==Already measured== | ==Already measured== | ||
*Q04400 - Our standard inverter. Works reliably (quantitatively), switches at low input. | *Q04400 - Our standard inverter. Works reliably (quantitatively), switches at low input. | ||
Revision as of 13:30, 30 June 2006
stuff...
- tetR QPI
Already measured
- Q04400 - Our standard inverter. Works reliably (quantitatively), switches at low input.
- Q03400 - tetR inverter, weaker RBS driving tetR. Looks like it starts to switch, but very high GFP gives very high RFP numbers. Unclear what's happening.
- Q01400 - tetR inverter, weakest RBS. Generally looks like it's stuck in the high-output stage. Some evidence suggests it might be switching, however.
- Q01530 - p22 cII inverter. Appears to be stuck in the low-output stage, perhaps due to crosstalk with CW2553/lambda.
- Q04530 - Same as Q01530, but with a stronger RBS driving the cII. Also stuck in low-output.
- Q04740 - penI inverter with strongest RBS. Works well, switches at higher input than Q04400.
- Q02740 - penI inverter, strong RBS. Looks like it begins to switch at high input.
- Q03740 - penI inverter, medium strength RBS. Stuck on.
- Q04400.007 - mutated Q04400, point mutation in RBS. Works well, switches later than Q04400.
To be measured/constructed/etc
- Q04720 - mnt inverter, strong repressor
- Q04730 - mnt inverter, weak repressor
- Q04400.007.Q04740/Q04740.Q04400.007 - possible bistable switches
- Q04510 - Ligated into the screening plasmid, but somehow missed being characterized. Seems unlikely to work, but hasn't been tested.
- Q07400/Q08400/Q09400 - tetR inverters using RBS's from the Voigt lab. Not BB'd, and previous attempts were unsuccessful.
