Endy:Screening plasmid/Inverter characterization: Difference between revisions

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==Already measured==
*Q04400.007 - mutated Q04400 (tetR QPI), point mutation in RBS.  Works well, switches later than Q04400.
[[Image:June22_2006_tetR_QPI_rep1.png|tetR QPI]]
*Q04400 - Works reliably (quantitatively), switches at low input.
*Q03400 - tetR inverter, weaker RBS driving tetR. Looks like it starts to switch, but very high GFP gives very high RFP numbers. Unclear what's happening.
*Q01400 - tetR inverter, weakest RBS. Generally looks like it's stuck in the high-output stage. Some evidence suggests it might be switching, however.
*Q01530 - p22 cII inverter. Appears to be stuck in the low-output stage, perhaps due to crosstalk with CW2553/lambda.
*Q04530 - Same as Q01530, but with a stronger RBS driving the cII. Also stuck in low-output.
*Q04740 - penI inverter with strongest RBS.  Works well, switches at higher input than Q04400.
*Q02740 - penI inverter, strong RBS.  Looks like it begins to switch at high input.
*Q03740 - penI inverter, medium strength RBS.  Stuck on.


==To be measured/constructed/etc==
*Q04720 - mnt inverter, strong repressor
*Q04730 - mnt inverter, weak repressor
*Q04400.007.Q04740/Q04740.Q04400.007 - possible bistable switches
*Q04510 - Ligated into the screening plasmid, but somehow missed being characterized. Seems unlikely to work, but hasn't been tested.
*Q07400/Q08400/Q09400 - tetR inverters using RBS's from the Voigt lab. Not BB'd, and previous attempts were unsuccessful.

Revision as of 13:48, 30 June 2006