Endy:Screening plasmid/Inverter characterization: Difference between revisions
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*Q04400.007 - mutated Q04400 (tetR QPI), point mutation in RBS. Works well, switches later than Q04400. | *Q04400.007 - mutated Q04400 (tetR QPI), point mutation in RBS. Works well, switches later than Q04400. | ||
[[Image:June22_2006_tetR_QPI_rep1.png|tetR QPI]] | [[Image:June22_2006_tetR_QPI_rep1.png|tetR QPI]] | ||
*Q04720 - mnt inverter, strong repressor - output not very high, and doesn't appear to be doing much ([http://openwetware.org/wiki/Image:June29_2006_Q04720_MOFLO.png picture]) | |||
*Q04730 - mnt inverter, weak repressor - output not very high, and doesn't appear to be doing much ([http://openwetware.org/wiki/Image:June29_2006_Q04730mut_MOFLO.png picture]) | |||
==Measured with older versions of screening plasmid (I13537.pSB1A3, I13538.pSB1A3, or I13534.pSB4A3)== | ==Measured with older versions of screening plasmid (I13537.pSB1A3, I13538.pSB1A3, or I13534.pSB4A3)== | ||
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==To be measured/constructed/etc== | ==To be measured/constructed/etc== | ||
*Q04400.007.Q04740/Q04740.Q04400.007 - possible bistable switch - having trouble getting it cloned in the first direction (tetR-penI) - weird stuff happening with growth rate and trying to prep the final plasmid, though colony PCR looks fine - load issues? | |||
*Q04400.007.Q04740/Q04740.Q04400.007 - possible bistable | |||
*Q04510 - Ligated into the screening plasmid, but somehow missed being characterized. Seems unlikely to work, but hasn't been tested. | *Q04510 - Ligated into the screening plasmid, but somehow missed being characterized. Seems unlikely to work, but hasn't been tested. | ||
*Q07400/Q08400/Q09400 - tetR inverters using RBS's from the Voigt lab. Not BB'd, and previous attempts were unsuccessful. | *Q07400/Q08400/Q09400 - tetR inverters using RBS's from the Voigt lab. Not BB'd, and previous attempts were unsuccessful. | ||
Revision as of 14:20, 10 July 2006
Already measured with SP1.0 (I13534.pSB1A3)
- Q04400.007 - mutated Q04400 (tetR QPI), point mutation in RBS. Works well, switches later than Q04400.
- Q04720 - mnt inverter, strong repressor - output not very high, and doesn't appear to be doing much (picture)
- Q04730 - mnt inverter, weak repressor - output not very high, and doesn't appear to be doing much (picture)
Measured with older versions of screening plasmid (I13537.pSB1A3, I13538.pSB1A3, or I13534.pSB4A3)
- Q04400 - Works reliably (quantitatively), switches at low input.
- Q03400 - tetR inverter, weaker RBS driving tetR. Looks like it starts to switch, but very high GFP gives very high RFP numbers. Unclear what's happening.
- Q01400 - tetR inverter, weakest RBS. Generally looks like it's stuck in the high-output stage. Some evidence suggests it might be switching, however.
- Q01530 - p22 cII inverter. Appears to be stuck in the low-output stage, perhaps due to crosstalk with CW2553/lambda.
- Q04530 - Same as Q01530, but with a stronger RBS driving the cII. Also stuck in low-output.
- Q04740 - penI inverter with strongest RBS. Works well, switches at higher input than Q04400.
- Q02740 - penI inverter, strong RBS. Looks like it begins to switch at high input.
- Q03740 - penI inverter, medium strength RBS. Stuck on.
To be measured/constructed/etc
- Q04400.007.Q04740/Q04740.Q04400.007 - possible bistable switch - having trouble getting it cloned in the first direction (tetR-penI) - weird stuff happening with growth rate and trying to prep the final plasmid, though colony PCR looks fine - load issues?
- Q04510 - Ligated into the screening plasmid, but somehow missed being characterized. Seems unlikely to work, but hasn't been tested.
- Q07400/Q08400/Q09400 - tetR inverters using RBS's from the Voigt lab. Not BB'd, and previous attempts were unsuccessful.
