Endy:Screening plasmid/Inverter characterization

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Already measured

  • Q04400 - Our standard inverter. Works reliably (quantitatively).
  • Q03400 - tetR inverter, weaker RBS driving tetR. Looks like it starts to switch, but very high GFP gives very high RFP numbers. Unclear what's happening.
  • Q01400 - tetR inverter, weakest RBS. Generally looks like it's stuck in the high-output stage. Some evidence suggests it might be switching, however.
  • Q01530 - p22 cII inverter. Appears to be stuck in the low-output stage, perhaps due to crosstalk with CW2553/lambda.
  • Q04530 - Same as Q01530, but with a stronger RBS driving the cII. Also stuck in low-output.

To be measured/constructed/etc

  • Q04510 - Ligated into the screening plasmid, but somehow missed being characterized. Seems unlikely to work, but hasn't been tested.
  • Q02740/Q03740/Q04740 - PenI based inverters. Currently being constructed.
  • Q07400/Q08400/Q09400 - tetR inverters using RBS's from the Voigt lab. Not BB'd, and previous attempts were unsuccessful.
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