Endy:Screening plasmid/Notebook

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Contents

Approach

  1. Part 1
    • Construction of High BG-promoter RFP and Hairpin constructs(completed)
    • Characterization of Inducible GFP (spec out exps on plate reader / LH-FACS)
  2. Part 2
    • Characterization of Inducible RFP (same as inducible GFP exps)
    • High BG RFP-promoter and Hairpin experiments (completed) (Results: HighBG-promoter,Hairpin)
    • Based on results from (2) test inducible RFP (<bbpart>I13520</bbpart>) at low and high induction on MOFLO
  3. Part 3
    • Rebuild ESP if necessary based on High BG RFP/Hairpin
    • Test ESP (same as inducible GFP exps)
      • Test the flipped FP constructs, cassie's constructs, and also the other version of the ESP where we cut out ccdb and re-ligated.

Current projects

High BG RFP

Hairpin effectiveness

Characterization of Inducible GFP

Characterization of Inducible RFP

<bbpart>I13520</bbpart>

Characterization of Empty Screening Plasmid 1.0

To ensure our screening system was operating as expected we measured the expression levels of GFP and mRFP1 when the plasmid did not contain a part (e.g. input equals output).

Glycerol Boxes

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