Endy:Screening plasmid/RBS library: Difference between revisions
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(New page: ==Design== Can vary the Shine-Dalgarno (SD) sequence or the spacing between the SD and the start codon. If we're going to use oligo synthesis it will be easier to vary the SD to make a ve...) |
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Can vary the Shine-Dalgarno (SD) sequence or the spacing between the SD and the start codon. If we're going to use oligo synthesis it will be easier to vary the SD to make a very large library. Alternatively we could make a small library with different spacing by simply synthesizing each one. | Can vary the Shine-Dalgarno (SD) sequence or the spacing between the SD and the start codon. If we're going to use oligo synthesis it will be easier to vary the SD to make a very large library. Alternatively we could make a small library with different spacing by simply synthesizing each one. | ||
==Experimental approach== | |||
#Use Heather's library | #Use Heather's library | ||
#*This might be an easy way to get a library of RBSs that are known to work (and in this case ready to be characterized as well). | #*This might be an easy way to get a library of RBSs that are known to work (and in this case ready to be characterized as well). | ||
#Synthesize an oligo library (would only vary the SD in this case, not the spacing). | #Synthesize an oligo library (would only vary the SD in this case, not the spacing). | ||
Revision as of 15:00, 24 April 2007
Design
Can vary the Shine-Dalgarno (SD) sequence or the spacing between the SD and the start codon. If we're going to use oligo synthesis it will be easier to vary the SD to make a very large library. Alternatively we could make a small library with different spacing by simply synthesizing each one.
Experimental approach
- Use Heather's library
- This might be an easy way to get a library of RBSs that are known to work (and in this case ready to be characterized as well).
- Synthesize an oligo library (would only vary the SD in this case, not the spacing).
