Endy:Standard for composable inverters/Repressor kinetics

The kinetics of the available repressors is important for determining the expected chassis load required to operate an inverter based on the repressor. At a minimum, need to estimate the # molecules of repressor / copy of promoter needed for "full" repression.

Inverter characteristics

I/O

• Unrepressed promoter strength
• Fully repressed promoter strength (leakiness)
• Cooperativity

Load

• Number of molecules (or conc) of repressor for full repression
• Number of molecules (or conc) of repressor for ½ max repression

cI/pR

• There are references listed in the Sup Mat of the elowitz GRF paper that seem to have most of the numbers we need here.

tetR

tetR promoter tuning