Endy:Standard transformation positive control: Difference between revisions
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We are using pUC18 at a concentration of 1ng/uL and adding 1uL as a positive control. If you are interested in getting some of the DNA, ask [[Jason Kelly|Jason]]. A few of us will try storing our efficiency results here to collect some data on how we're doing, and identify any new appraoches that improve efficiency. | We are using [http://invitrogen.com/search/index.cfm?fuseaction=google.search&searchTerm=pUC18&num=10&category=Vectors pUC18] at a concentration of 1ng/uL and adding 1uL as a positive control. If you are interested in getting some of the DNA, ask [[Jason Kelly|Jason]]. A few of us will try storing our efficiency results here to collect some data on how we're doing, and identify any new appraoches that improve efficiency. | ||
==Procedure for measuring effiency== | ==Procedure for measuring effiency== | ||
Revision as of 17:14, 27 July 2006
We are using pUC18 at a concentration of 1ng/uL and adding 1uL as a positive control. If you are interested in getting some of the DNA, ask Jason. A few of us will try storing our efficiency results here to collect some data on how we're doing, and identify any new appraoches that improve efficiency.
Procedure for measuring effiency
- Plate on LB (non-selective) to count the total number of survivors of the transformation procedure.
- Plate on LB+amp (selective) to count the number of successful transformants.
- The dilution to plate will depend on the number of cells in your competent cell sample. If you know the number of cells in your competent cell sample you could also report the fraction of survivors.
