Endy:Victor3 Absorbance and fluorescence backgrounds

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Sample Absorbance GFP-Separated
Empty well 0.032 941
Water 0.042 924
EZ media + 0.4% Glycerol 0.038 1035
EZ media + 0.4% Glycerol + Amp 0.04 1003
EZ media + 0.4% Glycerol + Tet 0.047 943
EZ media + 0.4% Glycerol + Amp + Tet 0.048 978
EZ media + 0.4% Glucose + Amp + Tet 0.047 1708
EZ media + 0.4% Glycerol + Kan 0.046 833
EZ media + 0.4% Glycerol + Strep 0.055 738


Plot of media fluorescence (measured using GFP filters) versus time
Plot of media fluorescence (measured using GFP filters) versus time

This plot shows the background fluorescence of EZ rich media from Teknova + 0.4% glycerol + antibiotic over time. This is data from two separate runs done on different days (circles and squares). Each run is an average of 3 replicates.

This experiment was done on the Endy lab Victor3 plate reader using 96 well plates from the Endy lab but using media and antibiotic from the Knight Lab. Water was injected into the wells over the course of the experiment to counteract evaporation, according to this protocol.

The reason for the lower background of EZ rich media + streptomycin for one of the days is unknown.

This plot is intended to demonstrate fluctuation in background (media-only) fluorescence measurements over time. This data is very preliminary and has not been independently verified. Use at your own risk!

Plot of media absorbance versus time
Plot of media absorbance versus time

This plot shows the background absorbance of EZ rich media from Teknova + 0.4% glycerol + antibiotic over time. This is data from two separate runs done on different days (circles and squares). Each run is an average of 3 replicates.

This experiment was done on the Endy lab Victor3 plate reader using 96 well plates from the Endy lab but using media and antibiotic from the Knight Lab. Water was injected into the wells over the course of the experiment to counteract evaporation, according to this protocol.

This plot is intended to demonstrate fluctuation in background (media-only) absorbance measurements over time. This data is very preliminary and has not been independently verified. Use at your own risk!

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