Endy:Victor3 counteracting evaporation in long time courses
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Source
- Rosenfeld et al. J. Mol. Biol. (2002) 323, 5, pp785-93.
Protocol
- Add an injection step into the Victor protocol to inject 12ul of DI water into each well every 25mins. Or add water at any time interval that leads to a rate of .48ul/min.
- I believe this is a slightly faster rate of addition than used in the article above.
Notes
- This can keep well volume constant over a 10 hour protocol at 37degrees.
- There may be some variability depending on machine model so it is best to check or experiment with this rate of water addition.
- I have not used the method at temperatures other than 37degrees.
- The software will not allow you to dispense less than 5ul per step, so the minimal interval for adding water is ~10mins.
- Note that taking measurements (particularly several measurements over many wells) can take a significant amount of time, on the order of ~5mins. Adjust the shaking time or dispenser volume as needed.
- The software will try to prevent you from dispensing more than 250μl per well per experiment. This is because it thinks that any more will cause the wells on the plates we use to overflow. You can get around this by not selecting any wells to be measured when you save the protocol. This prevents the software from checking how much water you are going to dispense over the course of the experiment. So only choose the wells to be measured using the protocol wizard before starting your run
