Endy:Victor3 lamp energy

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Background

When defining a label on the Endy:Victor3 Plate Reader, it is possible to vary the lamp energy used for excitation. It was thought that increasing this energy might give higher signal to noise ratios and generally increase signal amplification.

Test Labels

To test this hypothesis, a number of labels were created that used identical parameters (485nm excitation, 535/25nm emission, top counting, normal apertures, 0.1s measurement time) but varied the lamp energy over almost the full range (0-65000).

Test Samples

A stationary phase overnight of I7101.4A3 in MG1655 and untransformed MG1655 in supplemented M9 were tested along with a supplemented M9 media blank.

Meausurements

The ratios of transformed to untransformed (GFP/No GFP) and transformed to plain media (Signal/Noise) are plotted below for the range of lamp energies tested.

Signal to noise ratios for varying lamp energies

Discussion

This data suggests that, under the conditions tested, signal to noise ratios cannot be improved significantly by varying lamp energies.

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