Endy:Victor3 plate reader
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| - | == | + | ==Introduction== |
| + | The Endy lab has a [http://las.perkinelmer.com/Catalog/default.htm?CategoryID=VICTOR%3Csup%3E3%3C%2Fsup%3E Perkin Elmer Victor3] plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact [[Barry Canton|Barry]]. The machine can make the following classes of measurement - | ||
| + | *Time-resolved fluorometry | ||
| + | *Fluorometry | ||
| + | *[[Endy:Victor3 plate reader/Absorbance labels|Photometry]] | ||
| + | *Luminometry | ||
| + | *Fluorescence polarization | ||
| + | *LANCE | ||
| + | We have a variety of filters to be used with these measurements, they are described [[Endy:Victor3 plate reader/filters|here]]. If you haven't used the machine before, have a look at this [[Endy:Victor3 Getting Started|overview of using the plate reader]]. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome. | ||
| - | + | ==Plate reader schedule== | |
| + | <html> | ||
| + | <center> | ||
| + | <iframe src="http://www.google.com/calendar/embed?src=endy_plate_reader%40mit.edu&title=Plate%20Reader%20Signup&chrome=NAVIGATION&height=588" style=" border-width:0 " width="640" frameborder="0" height="630"></iframe> | ||
| + | </center> | ||
| + | </html> | ||
| + | [[Endy:Victor3 plate reader/Schedule Access|How to sign up]] | ||
| - | == | + | ==Protocols== |
| + | ===Programming a basic protocol=== | ||
| + | [[Endy:Victor3 plate reader/protocol editing | Protocol editing]] | ||
| - | [[Endy:Victor3 detection limits and linear range|Detection limits and linear range]] | + | ===Creating and choosing labels=== |
| - | + | [[Endy:Victor3 plate reader/labels|What is a label?]]<br> | |
| - | [[Endy:Victor3 lamp energy| | + | [[Endy:Victor3 plate reader/detection limits and linear range|Detection limits and linear range]]<br> |
| - | + | [[Endy:Victor3 lamp energy|Setting an appropriate lamp energy]]<br> | |
| - | [[Endy:Victor3 | + | [[Endy:Victor3 plate reader/Absorbance labels|Absorbance labels]]<br> |
| + | [[Endy:Victor3 plate reader/Changing filter slides|Changing filter slides]]<br> | ||
| + | ===Running a protocol=== | ||
[[Endy:Victor3 arranging samples|Arranging samples]] | [[Endy:Victor3 arranging samples|Arranging samples]] | ||
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[[Endy:Victor3 counteracting evaporation in long time courses|Counteracting evaporation in long time courses]] | [[Endy:Victor3 counteracting evaporation in long time courses|Counteracting evaporation in long time courses]] | ||
| - | ==Data | + | ==Data Analysis== |
[[Endy:Basic data analysis on a plate reader|Basic data analysis]]<br> | [[Endy:Basic data analysis on a plate reader|Basic data analysis]]<br> | ||
| - | [[Endy:Measuring PoPS on a plate reader|Measuring PoPS]] | + | [[Endy:Victor3 Calculating fluorescent protein synthesis|Calculating fluorescent protein synthesis]]<br> |
| + | [[Endy:Measuring PoPS on a plate reader|Measuring PoPS]]<br> | ||
| + | [[Endy:Victor3 Absorbance and fluorescence backgrounds|Background measurements]]<br> | ||
| + | [[Endy:Victor3 plate reader/Measurement variation|Measurement variation]]<br> | ||
| + | [[Endy:Victor3 plate variation| Plate variation]]<br> | ||
| + | [[Endy:Victor3 Anecdotal| Anecdotal]]<br> | ||
| + | [[Endy:Victor3 absorbance labels|Converting from absorbance to OD600]]<br> | ||
| + | [[Endy:Victor3 plate reader/Data analysis tools|Data analysis tools]]<br> | ||
==When things go bad== | ==When things go bad== | ||
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[[Category: Equipment]] | [[Category: Equipment]] | ||
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Current revision
IntroductionThe Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement -
We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome. Plate reader schedule
ProtocolsProgramming a basic protocolCreating and choosing labelsWhat is a label? Running a protocolCounteracting evaporation in long time courses Data AnalysisBasic data analysis When things go bad
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