Endy:Victor3 plate reader

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(Data Analysis)
(Introduction)
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*LANCE
*LANCE
We have a variety of filters to be used with these measurements, they are described [[Endy:Victor3 plate reader/filters|here]].  If you haven't used the machine before, have a look at this [[Endy:Victor3 Getting Started|overview of using the plate reader]].  If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below.  All contributions are welcome.
We have a variety of filters to be used with these measurements, they are described [[Endy:Victor3 plate reader/filters|here]].  If you haven't used the machine before, have a look at this [[Endy:Victor3 Getting Started|overview of using the plate reader]].  If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below.  All contributions are welcome.
 +
 +
==Plate reader sign-up sheet==
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We're testing a google calendar as a replacement for the old paper sign-up sheet.  You can access the sign-up sheet in
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[http://www.google.com/calendar/ical/do3vfe9fl5osapua1d6avdnl5k@group.calendar.google.com/public/basic iCal format] or [http://www.google.com/calendar/feeds/do3vfe9fl5osapua1d6avdnl5k@group.calendar.google.com/public/basic xml format].  If you have a gmail account, send your username to [[Barry Canton|Barry]] to gain edit access to the sign-up sheet.  If you don't have a gmail account, you can still view the calendar and email [[Barry Canton|Barry]] with the times for which you would like to sign up.
==Protocols==
==Protocols==

Revision as of 21:03, 9 May 2006

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Contents

Introduction

The Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement -

  • Time-resolved fluorometry
  • Fluorometry
  • Photometry
  • Luminometry
  • Fluorescence polarization
  • LANCE

We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome.

Plate reader sign-up sheet

We're testing a google calendar as a replacement for the old paper sign-up sheet. You can access the sign-up sheet in iCal format or xml format. If you have a gmail account, send your username to Barry to gain edit access to the sign-up sheet. If you don't have a gmail account, you can still view the calendar and email Barry with the times for which you would like to sign up.

Protocols

Creating a basic protocol

Protocol editing

Creating and choosing labels

What is a label?
Detection limits and linear range
Setting an appropriate lamp energy
Absorbance labels

Running a protocol

Arranging samples

Counteracting evaporation in long time courses

Data Analysis

Basic data analysis
Measuring PoPS
Background measurements
Measurement variation
Plate variation
Anecdotal
Converting from absorbance to OD600
Data analysis tools

When things go bad

  • Instrument serial number - 4206012
  • Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support.
  • Endy:Victor3 Service History
Personal tools