Endy:Victor3 plate reader: Difference between revisions
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*Time-resolved fluorometry | *Time-resolved fluorometry | ||
*Fluorometry | *Fluorometry | ||
*Photometry | *[[Endy:Victor3 plate reader/Absorbance labels|Photometry]] | ||
*Luminometry | *Luminometry | ||
*Fluorescence polarization | *Fluorescence polarization | ||
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==Plate reader schedule== | ==Plate reader schedule== | ||
<html> | <html> | ||
<iframe src="http://www.google.com/calendar/embed?src=endy_plate_reader%40mit.edu&title=Plate%20Reader%20Signup&chrome=NAVIGATION&height=588" style=" border-width:0 " width="640" frameborder="0" height=" | <center> | ||
<iframe src="http://www.google.com/calendar/embed?src=endy_plate_reader%40mit.edu&title=Plate%20Reader%20Signup&chrome=NAVIGATION&height=588" style=" border-width:0 " width="640" frameborder="0" height="630"></iframe> | |||
</center> | |||
</html> | </html> | ||
[[Endy:Victor3 plate reader/Schedule Access|How to sign up]] | |||
==Protocols== | ==Protocols== | ||
=== | ===Programming a basic protocol=== | ||
[[Endy:Victor3 plate reader/protocol editing | Protocol editing]] | [[Endy:Victor3 plate reader/protocol editing | Protocol editing]] | ||
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[[Endy:Victor3 lamp energy|Setting an appropriate lamp energy]]<br> | [[Endy:Victor3 lamp energy|Setting an appropriate lamp energy]]<br> | ||
[[Endy:Victor3 plate reader/Absorbance labels|Absorbance labels]]<br> | [[Endy:Victor3 plate reader/Absorbance labels|Absorbance labels]]<br> | ||
[[Endy:Victor3 plate reader/Changing filter slides|Changing filter slides]]<br> | |||
===Running a protocol=== | ===Running a protocol=== | ||
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==Data Analysis== | ==Data Analysis== | ||
[[Endy:Basic data analysis on a plate reader|Basic data analysis]]<br> | [[Endy:Basic data analysis on a plate reader|Basic data analysis]]<br> | ||
[[Endy:Victor3 Calculating fluorescent protein synthesis|Calculating fluorescent protein synthesis]]<br> | |||
[[Endy:Measuring PoPS on a plate reader|Measuring PoPS]]<br> | [[Endy:Measuring PoPS on a plate reader|Measuring PoPS]]<br> | ||
[[Endy:Victor3 Absorbance and fluorescence backgrounds|Background measurements]]<br> | [[Endy:Victor3 Absorbance and fluorescence backgrounds|Background measurements]]<br> | ||
Latest revision as of 11:38, 29 October 2007
IntroductionThe Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement -
We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome. Plate reader schedule<html> <center> <iframe src="http://www.google.com/calendar/embed?src=endy_plate_reader%40mit.edu&title=Plate%20Reader%20Signup&chrome=NAVIGATION&height=588" style=" border-width:0 " width="640" frameborder="0" height="630"></iframe> </center> </html> How to sign up ProtocolsProgramming a basic protocolCreating and choosing labelsWhat is a label? Running a protocolCounteracting evaporation in long time courses Data AnalysisBasic data analysis When things go bad
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