Endy:Victor3 plate reader

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Introduction The Endy lab has a Perkin Elmer Victor3 plate reader with 2 dispenser pumps. If you are interested in trying our plate reader or finding out more about it, contact Barry. The machine can make the following classes of measurement - Time-resolved fluorometry Fluorometry Photometry Luminometry Fluorescence polarization LANCE We have a variety of filters to be used with these measurements, they are described here. If you haven't used the machine before, have a look at this overview of using the plate reader. If you have used this machine or similar machines, have a look at some of the usage notes and data analysis pages listed below. All contributions are welcome. Plate reader sign-up We're testing a google calendar as a replacement for the old paper sign-up sheet. The calendar name is Endy Plate Reader Sign Up. You can access the sign-up sheet in a couple of ways - If you are a google calendar user, search public calendars for "Endy Plate Reader Sign Up" and subscribe to it. If you send me your username, I will give you edit access. If you don't have a google calendar account, you can sign up using your MIT email address here If you use a different calendar application or RSS feed reader, you can subscribe to the ical file or the xml feed that are linked below although that won't let you edit the calendar. iCal link xml link The calendar should be open on the desktop of the plate reader computer. Protocols Creating a basic protocol Protocol editing Creating and choosing labels What is a label? Detection limits and linear range Setting an appropriate lamp energy Absorbance labels Running a protocol Arranging samples Counteracting evaporation in long time courses Data Analysis Basic data analysis Measuring PoPS Background measurements Measurement variation Plate variation Anecdotal Converting from absorbance to OD600 Data analysis tools When things go bad Instrument serial number - 4206012 Perkin Elmer Contact - 8007624000 (add a 91 prefix from an MIT phone). Choose option 3 and option 3 again to talk to tech. support. Endy:Victor3 Service History