Endy:Victor3 plate reader/Plates: Difference between revisions
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==Path length as a function of volume== | ==Path length as a function of volume added to a well== | ||
[[Image:WellVolume.png|thumb| | Calculated from the volume of a frustrum using the plate dimensions given in the link above. | ||
[[Image:WellVolume.png|thumb|right|300px]] | |||
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Converting absorbance of a 200μl sample to OD600 using the path length calculated above yields very different numbers than when using a calibration produced from samples measured in both the [[Endy:Portable spec|Endy Lab portable spec]] and the plate reader. | |||
Latest revision as of 15:49, 17 November 2007
The Endy Lab uses plates made by Greiner Bio One.
- Ordering page from ISC BioExpress. We get catalog no. T-3026-16
- Manufacturers product page.
- Plate and well dimensions (.pdf).
Path length as a function of volume added to a well
Calculated from the volume of a frustrum using the plate dimensions given in the link above.
| Volume (µl) | Path Length (cm) |
|---|---|
| 50 | 0.15 |
| 100 | 0.29 |
| 150 | 0.44 |
| 200 | 0.58 |
| 250 | 7.2 |
| 300 | 0.85 |
| 350 | 0.99 |
| 400 | 1.12 |
Converting absorbance of a 200μl sample to OD600 using the path length calculated above yields very different numbers than when using a calibration produced from samples measured in both the Endy Lab portable spec and the plate reader.
