# Endy:Victor3 plate reader/detection limits and linear range

(Difference between revisions)
 Revision as of 13:18, 7 May 2005 (view source)← Previous diff Revision as of 13:19, 7 May 2005 (view source)Next diff → Line 1: Line 1: Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results.  Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits. Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results.  Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits. - *Fluorescein MDL (Minimum detectable level) - 2fMoles/well.  Using 200$\mu$l per well this corresponds to 10pM + *Fluorescein MDL (Minimum detectable level) - 2fMoles/well.  Using 200$\mu$l per well this corresponds to 10pM. - *([http://www.perkinelmer.com Perkin Elmer]) believe the machine has a five decade linear range.  This means concentrations up to $\mu$M should be read linearly. + *([http://www.perkinelmer.com Perkin Elmer]) believe the machine has a five decade linear range.  This means concentrations up to $\mu$Molar should be read linearly. *For higher concentrations, use a smaller volume. *For higher concentrations, use a smaller volume. - *The emission of Fluorescein relative to GFP is not yet known.  We also do not know how the emission of purified fluorophores relates to the emission of fluorophores ''in vivo'' + *The emission of Fluorescein relative to GFP is not yet known.  We also do not know how the emission of purified fluorophores relates to the emission of fluorophores ''in vivo''.