Endy:Victor3 plate reader/detection limits and linear range - Revision history

Barry Canton at 23:13, 6 January 2006

2006-01-06T23:13:09Z

←Older revision		Revision as of 23:13, 6 January 2006
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-	~~Also see: [[Endy:Victor3 plate reader\|Victor3 plate reader]]~~
-
	==Absorbance measurements==		==Absorbance measurements==
	[[Image:TableSpec to Plate Reader.png\|thumb\|200px\|right\|Instrument reading for a serial dilution of cell culture]]		[[Image:TableSpec to Plate Reader.png\|thumb\|200px\|right\|Instrument reading for a serial dilution of cell culture]]

Barry Canton at 23:12, 6 January 2006

2006-01-06T23:12:42Z

←Older revision		Revision as of 23:12, 6 January 2006
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	Also see: [[Endy:Victor3 plate reader\|Victor3 plate reader]]		Also see: [[Endy:Victor3 plate reader\|Victor3 plate reader]]

		+	==Absorbance measurements==
		+	[[Image:TableSpec to Plate Reader.png\|thumb\|200px\|right\|Instrument reading for a serial dilution of cell culture]]
		+	For the OD600 label, the machine appears to respond in a linear fashion over the range of absorbances shown in this figure. More details of the experiment can be found here. Better data over a larger range will follow shortly.
		+
		+	<br style="clear:both" />
		+	==Fluorescence measurements==
	Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits.		Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits.

Barry Canton: Endy:Victor3 detection limits and linear range moved to Endy:Victor3 plate reader/detection limits and linear range

2006-01-06T23:05:58Z

Endy:Victor3 detection limits and linear range moved to Endy:Victor3 plate reader/detection limits and linear range

←Older revision

Revision as of 23:05, 6 January 2006

Barry Canton at 00:40, 1 August 2005

2005-08-01T00:40:32Z

←Older revision		Revision as of 00:40, 1 August 2005
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-	Also see: [[Endy:Victor3 ~~Plate Reader~~\|Victor3 ~~Plate Reader~~]]	+	Also see: [[Endy:Victor3 plate reader\|Victor3 plate reader]]

	Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits.		Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits.

Barry Canton at 00:35, 1 August 2005

2005-08-01T00:35:37Z

←Older revision		Revision as of 00:35, 1 August 2005
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		+	Also see: [[Endy:Victor3 Plate Reader\|Victor3 Plate Reader]]
		+
	Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits.		Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits.

Barry Canton at 17:23, 7 May 2005

2005-05-07T17:23:48Z

←Older revision		Revision as of 17:23, 7 May 2005
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	*The emission of Fluorescein relative to GFP is not yet known. We also do not know how the emission of purified fluorophores relates to the emission of fluorophores ''in vivo''.		*The emission of Fluorescein relative to GFP is not yet known. We also do not know how the emission of purified fluorophores relates to the emission of fluorophores ''in vivo''.
		+
		+	*More to follow on this hopefully.

Barry Canton at 17:19, 7 May 2005

2005-05-07T17:19:50Z

←Older revision		Revision as of 17:19, 7 May 2005
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	Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits.		Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits.

-	*Fluorescein MDL (Minimum detectable level) - 2fMoles/well. Using 200<math>\mu</math>l per well this corresponds to 10pM	+	*Fluorescein MDL (Minimum detectable level) - 2fMoles/well. Using 200<math>\mu</math>l per well this corresponds to 10pM.

-	*([http://www.perkinelmer.com Perkin Elmer]) believe the machine has a five decade linear range. This means concentrations up to <math>\mu</math>M should be read linearly.	+	*([http://www.perkinelmer.com Perkin Elmer]) believe the machine has a five decade linear range. This means concentrations up to <math>\mu</math>Molar should be read linearly.

	*For higher concentrations, use a smaller volume.		*For higher concentrations, use a smaller volume.

-	*The emission of Fluorescein relative to GFP is not yet known. We also do not know how the emission of purified fluorophores relates to the emission of fluorophores ''in vivo''	+	*The emission of Fluorescein relative to GFP is not yet known. We also do not know how the emission of purified fluorophores relates to the emission of fluorophores ''in vivo''.

Barry Canton at 17:18, 7 May 2005

2005-05-07T17:18:46Z

←Older revision		Revision as of 17:18, 7 May 2005
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	*Fluorescein MDL (Minimum detectable level) - 2fMoles/well. Using 200<math>\mu</math>l per well this corresponds to 10pM		*Fluorescein MDL (Minimum detectable level) - 2fMoles/well. Using 200<math>\mu</math>l per well this corresponds to 10pM

-	*([http://www.perkinelmer.com Perkin Elmer]) believe the machine has a five decade linear range. This means concentrations up to <math>/mu</math>M should be read linearly.	+	*([http://www.perkinelmer.com Perkin Elmer]) believe the machine has a five decade linear range. This means concentrations up to <math>\mu</math>M should be read linearly.

	*For higher concentrations, use a smaller volume.		*For higher concentrations, use a smaller volume.

	*The emission of Fluorescein relative to GFP is not yet known. We also do not know how the emission of purified fluorophores relates to the emission of fluorophores ''in vivo''		*The emission of Fluorescein relative to GFP is not yet known. We also do not know how the emission of purified fluorophores relates to the emission of fluorophores ''in vivo''

Barry Canton at 17:18, 7 May 2005

2005-05-07T17:18:33Z

←Older revision		Revision as of 17:18, 7 May 2005
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-	Recent experiments have ~~brought~~ the ~~linear~~ range of the ~~machine into question~~. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate ~~readers~~ detection limits.	+	Recent experiments have raised the question of what range of concentrations can we expect the plate reader to give linear results. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate reader's detection limits.

	*Fluorescein MDL (Minimum detectable level) - 2fMoles/well. Using 200<math>\mu</math>l per well this corresponds to 10pM		*Fluorescein MDL (Minimum detectable level) - 2fMoles/well. Using 200<math>\mu</math>l per well this corresponds to 10pM

Barry Canton at 17:17, 7 May 2005

2005-05-07T17:17:22Z

New page

Recent experiments have brought the linear range of the machine into question. Chris Quinlan ([http://www.perkinelmer.com of Perkin Elmer]) provided the following information about the plate readers detection limits.

*Fluorescein MDL (Minimum detectable level) - 2fMoles/well. Using 200<math>\mu</math>l per well this corresponds to 10pM

*([http://www.perkinelmer.com Perkin Elmer]) believe the machine has a five decade linear range. This means concentrations up to <math>/mu</math>M should be read linearly.

*For higher concentrations, use a smaller volume.

*The emission of Fluorescein relative to GFP is not yet known. We also do not know how the emission of purified fluorophores relates to the emission of fluorophores ''in vivo''