Endy:Victor3 plate reader/detection limits and linear range

From OpenWetWare

< Endy:Victor3 plate reader
Revision as of 13:17, 7 May 2005 by Barry Canton (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Add to My Links

Recent experiments have brought the linear range of the machine into question. Chris Quinlan (of Perkin Elmer) provided the following information about the plate readers detection limits.

  • Fluorescein MDL (Minimum detectable level) - 2fMoles/well. Using 200μl per well this corresponds to 10pM
  • (Perkin Elmer) believe the machine has a five decade linear range. This means concentrations up to / muM should be read linearly.
  • For higher concentrations, use a smaller volume.
  • The emission of Fluorescein relative to GFP is not yet known. We also do not know how the emission of purified fluorophores relates to the emission of fluorophores in vivo
Personal tools