Endy:Victor3 plate reader/detection limits and linear range
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Recent experiments have brought the linear range of the machine into question. Chris Quinlan (of Perkin Elmer) provided the following information about the plate readers detection limits.
- Fluorescein MDL (Minimum detectable level) - 2fMoles/well. Using 200μl per well this corresponds to 10pM
- (Perkin Elmer) believe the machine has a five decade linear range. This means concentrations up to / muM should be read linearly.
- For higher concentrations, use a smaller volume.
- The emission of Fluorescein relative to GFP is not yet known. We also do not know how the emission of purified fluorophores relates to the emission of fluorophores in vivo