Endy:Victor3 plate reader/filters

From OpenWetWare

< Endy:Victor3 plate reader(Difference between revisions)
Jump to: navigation, search
(Emission Filters)
Current revision (12:55, 14 March 2007) (view source)
(Emission Filters)
 
Line 68: Line 68:
|510DF40EM|| ? ||510 ||40?||Uninstalled||||
|510DF40EM|| ? ||510 ||40?||Uninstalled||||
|-
|-
-
|510DF20EM|| GFP emission ||510 ||20?||B1||||
+
|510DF20EM|| GFP emission ||510 ||20?||B1|||| Used by Lauffenburger lab for both 505nm and 520nm fluorescence emission -- only okay.
|-
|-
|F0426A|| ||620||?||B2||No||We use this for RFP and mCherry emission.  Anecdotal evidence suggests that it isn't as bright as the standard GFP label we use.
|F0426A|| ||620||?||B2||No||We use this for RFP and mCherry emission.  Anecdotal evidence suggests that it isn't as bright as the standard GFP label we use.

Current revision

Excitation Filters

Name Use Transmission Peak Bandwidth Filter Wheel Factory Installed Use
P405Colorimetric label emission 405 10 A1 Yes Used by Lauffenburger lab for 400nm fluorescence excitation.
P450Colorimetric label emission 450 6 A6Yes
P490Colorimetric label emission 490 6 A3Yes Used by Nora in Keating lab for colometric assays to measure abs.
380DF 380 15 -- No?
P570 570 18 Uninstalled
595DF 595 10 --
D340TR fluorescence excitation 340 50 UninstalledYes
D320TR fluorescence excitation 320 80 --Yes
F485Fluorescein excitation 485 15 A5Yes We use this to excite GFP. Also used by Lauffenburger lab for 485nm fluorescence excitation.
F355 Umbelliferone excitation 355 40 UninstalledYes
RFP-544RFP excitation544?A2No
OD-600Absorbance600?A4NoWe use this for the OD600 label
YFPYFP excitation5008A7No
CFP (F0439A P430)CFP excitation430?A8NoThis is listed as a P430, don't know if this means we shouldn't use it as a fluorescent excitation filter.

Emission Filters

Name Use Transmission Peak Bandwidth Filter Slide Factory Installed Use
D615Europium chelate emission 615 8.5 A1Yes Used by Lauffenburger lab for 590nm fluorescence emission -- only okay, but better than RFP emission filter in slot B2 [F0426A 620nm].
D665Europium chelate emission 665 7.5 A8Yes
D642Samarium chelate emission 642 6.5 A2Yes
D545Terbium chelate emission 545 7.5 A3Yes
D572Dysprosium chelate emission 572 7.5 UninstalledYes
F535Fluorescein emission 535 25 A5Yes
F460 Umbelliferone emission 460 40 --Yes
620DF10EM ? ? ?Uninstalled
630DF10 ? ? ?Uninstalled
510DF40EM ? 510 40?Uninstalled
510DF20EM GFP emission 510 20?B1 Used by Lauffenburger lab for both 505nm and 520nm fluorescence emission -- only okay.
F0426A 620?B2NoWe use this for RFP and mCherry emission. Anecdotal evidence suggests that it isn't as bright as the standard GFP label we use.
F0436AYFP emission 535?B3NoWe also use this for GFP emission because we were picking up excitation light when using the 510 filter.
4D318 Corion 460?B4No
F0432A D572 572?B8No

Abbreviations:
D = Time resolved fluorometry, including LANCE
F = Fluorometry
P = Photometry
L = Luminometry
These are the recommended applications for the filters. It is not clear whether there is actually any difference between, for example, a fluorometry excitation filter and a photometry filter.

Personal tools