Endy:Victor3 plate variation: Difference between revisions
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[[Image:NewplateGFP|thumb|300px|left|New plate]] | [[Image:NewplateGFP|thumb|300px|left|New plate]] | ||
[[Image:OldplateGFP.jpg|thumb|300px|left|Old plate]] | [[Image:OldplateGFP.jpg|thumb|300px|left|Old plate]] | ||
==Conclusion== | |||
*The washed plate results in a higher average background reading. | |||
*The washed plate actually results in tighter distribution of readings. | |||
*For most applications, washed plates are perfectly acceptable. | |||
Revision as of 14:37, 8 August 2005
Background
96 well plates with transparent bases cost ~$4 each. It was unclear if washing plates (bleach, followed by ethanol) led to greater variation in readings.
Method
The GFP and absorbance of every well on a new and a washed plate were measured. The wells were empty.
Results
Histograms of the GFP readings for each plate are shown below.
Conclusion
- The washed plate results in a higher average background reading.
- The washed plate actually results in tighter distribution of readings.
- For most applications, washed plates are perfectly acceptable.
