Endy:Victor3 plate variation

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[[Image:NewplateGFP|thumb|300px|left|New plate]]
[[Image:NewplateGFP|thumb|300px|left|New plate]]
[[Image:OldplateGFP.jpg|thumb|300px|left|Old plate]]
[[Image:OldplateGFP.jpg|thumb|300px|left|Old plate]]
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==Conclusion==
==Conclusion==
*The washed plate results in a higher average background reading.
*The washed plate results in a higher average background reading.
*The washed plate actually results in tighter distribution of readings.
*The washed plate actually results in tighter distribution of readings.
*For most applications, washed plates are perfectly acceptable.
*For most applications, washed plates are perfectly acceptable.

Revision as of 16:37, 8 August 2005

Contents

Background

96 well plates with transparent bases cost ~$4 each. It was unclear if washing plates (bleach, followed by ethanol) led to greater variation in readings.

Method

The GFP and absorbance of every well on a new and a washed plate were measured. The wells were empty.

Results

Histograms of the GFP readings for each plate are shown below.

New plate
New plate
Old plate
Old plate

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Conclusion

  • The washed plate results in a higher average background reading.
  • The washed plate actually results in tighter distribution of readings.
  • For most applications, washed plates are perfectly acceptable.
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