Endy: Journal Club: Le et al 2005: Difference between revisions
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==Jounal Club Date== | ==Jounal Club Date== | ||
TODAY! In '''574''' at 2 pm. | |||
Voting's done. Friday, October 7th it is. Per suggestion, we will move the time back to accomodate Harvard Systems Bio lunch. I will try to reserve a room for 2 pm. -[[User:Cconboy|cmc]] 10:30, 3 Oct 2005 (EDT) | |||
===Friday, 10-07-05, | ===Friday, 10-07-05, 2pm, room 68-574=== | ||
Harvard Systems Biology lunch is from 12-1 pm. Should we delay this a bit in case people are returning from there? | |||
If people can do 1:30 equally well, then yes. [[User:Cconboy|cmc]] 19:43, 27 Sep 2005 (EDT) | |||
#[[Caitlin Conboy|Caitlin]] | |||
#[[Reshma Shetty|RS]] | |||
#[[Drew Endy]] | |||
#[[Samantha Sutton]] | |||
#[[User:Bcanton|BC]] | |||
#[[Jason Kelly]] - i'd be a little late due to systems bio lunch... | |||
#[[Austin Che]] | |||
#[[Heather Keller|HK]] | |||
==Reference== | ==Reference== | ||
Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9160-4. Epub 2005 Jun 20. | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15967986&query_hl=1 Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9160-4. Epub 2005 Jun 20.] | ||
Real-time RNA profiling within a single bacterium. | Real-time RNA profiling within a single bacterium. | ||
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Characterizing the dynamics of specific RNA levels requires real-time RNA profiling in a single cell. We show that the combination of a synthetic modular genetic system with fluorescence correlation spectroscopy allows us to directly measure in real time the activity of any specific promoter in prokaryotes. Using a simple inducible gene expression system, we found that induced RNA levels within a single bacterium of Escherichia coli exhibited a pulsating profile in response to a steady input of inducer. The genetic deletion of an efflux pump system, a key determinant of antibiotic resistance, altered the pulsating transcriptional dynamics and caused overexpression of induced RNA. In contrast with population measurements, real-time RNA profiling permits identifying relationships between genotypes and transcriptional dynamics that are accessible only at the level of the single cell. | Characterizing the dynamics of specific RNA levels requires real-time RNA profiling in a single cell. We show that the combination of a synthetic modular genetic system with fluorescence correlation spectroscopy allows us to directly measure in real time the activity of any specific promoter in prokaryotes. Using a simple inducible gene expression system, we found that induced RNA levels within a single bacterium of Escherichia coli exhibited a pulsating profile in response to a steady input of inducer. The genetic deletion of an efflux pump system, a key determinant of antibiotic resistance, altered the pulsating transcriptional dynamics and caused overexpression of induced RNA. In contrast with population measurements, real-time RNA profiling permits identifying relationships between genotypes and transcriptional dynamics that are accessible only at the level of the single cell. | ||
==Related resources== | |||
*[http://www.biophysics.org/education/schwille.pdf Fluorescence Correlation Spectroscopy: An Introduction to its Concepts and Applications] by Petra Schwille and Elke Haustein. A recommended introductory review on fluorescence correlation spectroscopy. | |||
Latest revision as of 08:33, 13 October 2005
Jounal Club Date
TODAY! In 574 at 2 pm.
Voting's done. Friday, October 7th it is. Per suggestion, we will move the time back to accomodate Harvard Systems Bio lunch. I will try to reserve a room for 2 pm. -cmc 10:30, 3 Oct 2005 (EDT)
Friday, 10-07-05, 2pm, room 68-574
Harvard Systems Biology lunch is from 12-1 pm. Should we delay this a bit in case people are returning from there? If people can do 1:30 equally well, then yes. cmc 19:43, 27 Sep 2005 (EDT)
- Caitlin
- RS
- Drew Endy
- Samantha Sutton
- BC
- Jason Kelly - i'd be a little late due to systems bio lunch...
- Austin Che
- HK
Reference
Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9160-4. Epub 2005 Jun 20.
Real-time RNA profiling within a single bacterium.
Le TT, Harlepp S, Guet CC, Dittmar K, Emonet T, Pan T, Cluzel P.
Institute for Biophysical Dynamics and The James Franck Institute and Department of Biochemistry and Molecular Biology, University of Chicago, 5640 South Ellis Avenue, Chicago, IL 60637, USA.
Characterizing the dynamics of specific RNA levels requires real-time RNA profiling in a single cell. We show that the combination of a synthetic modular genetic system with fluorescence correlation spectroscopy allows us to directly measure in real time the activity of any specific promoter in prokaryotes. Using a simple inducible gene expression system, we found that induced RNA levels within a single bacterium of Escherichia coli exhibited a pulsating profile in response to a steady input of inducer. The genetic deletion of an efflux pump system, a key determinant of antibiotic resistance, altered the pulsating transcriptional dynamics and caused overexpression of induced RNA. In contrast with population measurements, real-time RNA profiling permits identifying relationships between genotypes and transcriptional dynamics that are accessible only at the level of the single cell.
Related resources
- Fluorescence Correlation Spectroscopy: An Introduction to its Concepts and Applications by Petra Schwille and Elke Haustein. A recommended introductory review on fluorescence correlation spectroscopy.
