# Endy:dNTP stock solution

(Difference between revisions)
 Revision as of 16:50, 30 August 2005 (view source)← Previous diff Revision as of 09:04, 31 August 2005 (view source)Next diff → Line 5: Line 5: I don't know if there's any advantage other than to minimize freeze-thaw cycles to keeping intermediate dilutions of individual dNTPs rather than combining them immediately into the 10x solution. [[User:ClarkeS|Sean]] 17:50, 30 Aug 2005 (EDT) I don't know if there's any advantage other than to minimize freeze-thaw cycles to keeping intermediate dilutions of individual dNTPs rather than combining them immediately into the 10x solution. [[User:ClarkeS|Sean]] 17:50, 30 Aug 2005 (EDT) + + My dNTP stocks are: + *100x: 25mM each dNTP. Combine equal volumes each individual dNTP. + *10x: 2.5mM each dNTP. Dilute back 100x stock 1:10. + Working concentration: 250 μM
+ -[[User:Jkm|Jkm]] 10:04, 31 Aug 2005 (EDT)

## Revision as of 09:04, 31 August 2005

Each individual dNTP comes as 25 μmol in 100 mM solution. The lids are color-coded to each nucleic acid.

1. Dilute these in DI water to 2.5 mM to get four tubes of diluted dNTP, i.e., n*(1 μl dNTP + 39 μl water).
2. Combine these in equal amounts to yield a 10x solution for use in PCR.

I don't know if there's any advantage other than to minimize freeze-thaw cycles to keeping intermediate dilutions of individual dNTPs rather than combining them immediately into the 10x solution. Sean 17:50, 30 Aug 2005 (EDT)

My dNTP stocks are:

• 100x: 25mM each dNTP. Combine equal volumes each individual dNTP.
• 10x: 2.5mM each dNTP. Dilute back 100x stock 1:10.

Working concentration: 250 μM
-Jkm 10:04, 31 Aug 2005 (EDT)