# Endy:dNTP stock solution

(Difference between revisions)
 Revision as of 10:04, 31 August 2005 (view source)← Previous diff Revision as of 14:52, 31 August 2005 (view source)Next diff → Line 1: Line 1: Each individual dNTP comes as 25 μmol in 100 mM solution.  The lids are color-coded to each nucleic acid. Each individual dNTP comes as 25 μmol in 100 mM solution.  The lids are color-coded to each nucleic acid. + ==Protocol #1== #Dilute these in DI water to 2.5 mM to get four tubes of diluted dNTP, i.e., n*(1 μl dNTP + 39 μl water). #Dilute these in DI water to 2.5 mM to get four tubes of diluted dNTP, i.e., n*(1 μl dNTP + 39 μl water). #Combine these in equal amounts to yield a 10x solution for use in PCR. #Combine these in equal amounts to yield a 10x solution for use in PCR. - I don't know if there's any advantage other than to minimize freeze-thaw cycles to keeping intermediate dilutions of individual dNTPs rather than combining them immediately into the 10x solution. [[User:ClarkeS|Sean]] 17:50, 30 Aug 2005 (EDT) + I don't know if there's any advantage other than to minimize freeze-thaw cycles to keeping intermediate dilutions of individual dNTPs rather than combining them immediately into the 10x solution.
[[User:ClarkeS|Sean]] 17:50, 30 Aug 2005 (EDT) + ==Protocol #2== My dNTP stocks are: My dNTP stocks are: - *100x: 25mM each dNTP. Combine equal volumes each individual dNTP. + *100x: 25mM each dNTP. Combine equal volumes of 100 mM individual dNTPs. *10x: 2.5mM each dNTP. Dilute back 100x stock 1:10. *10x: 2.5mM each dNTP. Dilute back 100x stock 1:10. - Working concentration: 250 μM
+ *Working concentration: 250 μM
-[[User:Jkm|Jkm]] 10:04, 31 Aug 2005 (EDT) -[[User:Jkm|Jkm]] 10:04, 31 Aug 2005 (EDT)

## Revision as of 14:52, 31 August 2005

Each individual dNTP comes as 25 μmol in 100 mM solution. The lids are color-coded to each nucleic acid.

## Protocol #1

1. Dilute these in DI water to 2.5 mM to get four tubes of diluted dNTP, i.e., n*(1 μl dNTP + 39 μl water).
2. Combine these in equal amounts to yield a 10x solution for use in PCR.

I don't know if there's any advantage other than to minimize freeze-thaw cycles to keeping intermediate dilutions of individual dNTPs rather than combining them immediately into the 10x solution.
Sean 17:50, 30 Aug 2005 (EDT)

## Protocol #2

My dNTP stocks are:

• 100x: 25mM each dNTP. Combine equal volumes of 100 mM individual dNTPs.
• 10x: 2.5mM each dNTP. Dilute back 100x stock 1:10.
• Working concentration: 250 μM

-Jkm 10:04, 31 Aug 2005 (EDT)