Enzyme selection for BioBricks digest

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Line 3: Line 3:
*-----E--X---Part---S--P-----
*-----E--X---Part---S--P-----
-
Vector with upstream part - SP
+
Vector with upstream part - S,P
-
Downstream Insert - XP
+
Downstream Insert - X,P
-
Upstream Insert - ES
+
Upstream Insert - E,S
-
Vector with downstream part- EX
+
Vector with downstream part- E,X
 +
 
 +
 
 +
 
 +
 
 +
E= EcoRI
 +
X= XbaI
 +
S= SpeI
 +
P= PstI
 +
 
 +
==Notes==
 +
Very small (<100kb) inserts have shown to be difficult to remove from a gel purification.  It is recommended that during assembly very small pieces be kept on the vector and larger pieces be used as inserts if possible.

Revision as of 12:57, 20 June 2006

A BioBricks part:

  • -----E--X---Part---S--P-----

Vector with upstream part - S,P

Downstream Insert - X,P

Upstream Insert - E,S

Vector with downstream part- E,X



E= EcoRI X= XbaI S= SpeI P= PstI

Notes

Very small (<100kb) inserts have shown to be difficult to remove from a gel purification. It is recommended that during assembly very small pieces be kept on the vector and larger pieces be used as inserts if possible.

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