Enzyme selection for BioBricks digest: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Notes) |
No edit summary |
||
| Line 20: | Line 20: | ||
==Notes== | ==Notes== | ||
Very small (<100kb) inserts have shown to be difficult to remove from a gel purification. It is recommended that during assembly very small pieces be kept on the vector and larger pieces be used as inserts if possible. Otherwise just use 3A assembly or [[ | Very small (<100kb) inserts have shown to be difficult to remove from a gel purification. It is recommended that during assembly very small pieces be kept on the vector and larger pieces be used as inserts if possible. Otherwise just use [[Synthetic Biology:BioBricks/3A assembly|3A assembly]] or [[Amplified Insert Assembly]]. | ||
Revision as of 21:56, 18 March 2011
A BioBricks part:
- -----E--X---Part---S--P-----
Vector with upstream part - S,P
Downstream Insert - X,P
Upstream Insert - E,S
Vector with downstream part- E,X
E= EcoRI
X= XbaI
S= SpeI
P= PstI
Notes
Very small (<100kb) inserts have shown to be difficult to remove from a gel purification. It is recommended that during assembly very small pieces be kept on the vector and larger pieces be used as inserts if possible. Otherwise just use 3A assembly or Amplified Insert Assembly.
