Enzyme selection for BioBricks digest
A BioBricks part:
Vector with upstream part - S,P
Downstream Insert - X,P
Upstream Insert - E,S
Vector with downstream part- E,X
E= EcoRI X= XbaI S= SpeI P= PstI
Very small (<100kb) inserts have shown to be difficult to remove from a gel purification. It is recommended that during assembly very small pieces be kept on the vector and larger pieces be used as inserts if possible.