Enzyme selection for BioBricks digest

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A BioBricks part:

  • -----E--X---Part---S--P-----

Vector with upstream part - S,P

Downstream Insert - X,P

Upstream Insert - E,S

Vector with downstream part- E,X



E= EcoRI X= XbaI S= SpeI P= PstI

Notes

Very small (<100kb) inserts have shown to be difficult to remove from a gel purification. It is recommended that during assembly very small pieces be kept on the vector and larger pieces be used as inserts if possible.

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