Erickson:Colony PCR
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Colony PCR
- PCR Master Mix
1 μL Platinum Taq® 30 μL 10X PCR Buffer (-MgCl2) 5 μL Primers (forward and reverse) (10 μL total) 6 μL 50 mM MgCl2 5 uL PCR nucleotides 198 μL H2O 250 μL volume total. Adjust volume according to needs.
- For colony PCR, scrape small volume of colony and add to PCR Master Mix (20 μL)