Erickson:Protocols: Difference between revisions
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{{Template:Erickson lab}} | {{Template:Erickson lab}} | ||
[[Erickson: | ==PCR== | ||
[[Erickson:Colony PCR|Colony PCR]] | |||
<br> | <br> | ||
[[Erickson:REal-time PCR|Real-Time PCR]] | |||
<br> | |||
[[Erickson:PCR Clean-up for Sequencing|PCR Clean-up for Sequencing]] | |||
<br> | |||
[[Erickson:2-log Ladder|2-log Ladder]] | |||
==RNA== | |||
[[Erickson:RNA|RNA Isolation Protocol (TRIzol)]] | |||
[[Erickson:RNA Shredder|RNA Isolation Protocol (Mini Shredder)]] | |||
<br> | |||
==Fleas== | |||
[[Erickson:Flea|Flea Bedding]] | [[Erickson:Flea|Flea Bedding]] | ||
<br> | <br> | ||
[[Erickson:Flea Crushing|Flea Crushing]] | |||
<br> | |||
==Media== | |||
[[Erickson:LB (Agar)|LB mixture (includes plates mix)]] | [[Erickson:LB (Agar)|LB mixture (includes plates mix)]] | ||
<br> | <br> | ||
[[Erickson:LB Running Buffer|LB Buffer]] | [[Erickson:LB Running Buffer|LB Buffer]] | ||
<br> | |||
[[Erickson:TAE Buffer|TAE Buffer]] | |||
<br> | |||
[[Erickson:TB|TB]] | |||
<br> | |||
[[Erickson:Frozen Stocks|Frozen Stocks]] | |||
==Antibiotics== | |||
[[Erickson:Kanamycin|Kanamycin]] | |||
<br> | |||
[[Erickson:Carbenacillin|Carbenacillin]] | |||
<br> | |||
[[Erickson:Tetracycline|Tetracycline]] | |||
<br> | |||
[[Erickson:Irgasan|Irgasan]] | |||
<br> | |||
[[Erickson:Chloramphenicol|Chloramphenicol]] | |||
==Transposon Mutagenesis== | |||
[[Erickson:Tri-parental Mating|Tri-parental Mating]] | |||
<br> | |||
[[Erickson: Electroporation|Electroporation]] | |||
<br> | |||
[[Erickson: Bi-parental Mating|Bi-parental Mating]] | |||
<br> | |||
==Mutant Generation== | |||
[[Image:Making_a_mutant.pptx]] | |||
==Competent Cell Generation== | |||
[[Erickson:Competent Cell Generation|Competent Cells]] | |||
Latest revision as of 12:00, 10 November 2009
PCR
Colony PCR
Real-Time PCR
PCR Clean-up for Sequencing
2-log Ladder
RNA
RNA Isolation Protocol (TRIzol)
RNA Isolation Protocol (Mini Shredder)
Fleas
Media
LB mixture (includes plates mix)
LB Buffer
TAE Buffer
TB
Frozen Stocks
Antibiotics
Kanamycin
Carbenacillin
Tetracycline
Irgasan
Chloramphenicol
