Erickson:Protocols

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(Bacterial Conjugation)
(Transposon Mutagenesis)
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[[Erickson: Electroporation|Electroporation]]
[[Erickson: Electroporation|Electroporation]]
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[[Erickson: Bi-parental Mating|Bi-parental Mating]]
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Revision as of 14:19, 23 June 2008

Contents

PCR

Colony PCR

RNA

RNA Isolation Protocol (TRIzol)

RNA Isolation Protocol (Mini Shredder)

Fleas

Flea Bedding

Media

LB mixture (includes plates mix)
LB Buffer
TAE Buffer

Antibiotics

Kanamycin
Carbenacillin
Tetracycline
Irgasan
Chloramphenicol

Transposon Mutagenesis

Tri-parental Mating

Electroporation

Bi-parental Mating

Personal tools