Erickson:Protocols

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[[Erickson:TAE Buffer|TAE Buffer]]
[[Erickson:TAE Buffer|TAE Buffer]]
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[[Erickson:TB]]
==Antibiotics==
==Antibiotics==
[[Erickson:Kanamycin|Kanamycin]]
[[Erickson:Kanamycin|Kanamycin]]

Revision as of 19:23, 14 May 2009

Contents

PCR

Colony PCR
Real-Time PCR

RNA

RNA Isolation Protocol (TRIzol)

RNA Isolation Protocol (Mini Shredder)

Fleas

Flea Bedding

Media

LB mixture (includes plates mix)
LB Buffer
TAE Buffer
Erickson:TB

Antibiotics

Kanamycin
Carbenacillin
Tetracycline
Irgasan
Chloramphenicol

Transposon Mutagenesis

Tri-parental Mating

Electroporation

Bi-parental Mating

Mutant Generation

Image:Making a mutant.pptx

Competent Cell Generation

Competent Cells

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