Erickson:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 20: Line 20:
[[Erickson:TAE Buffer|TAE Buffer]]
[[Erickson:TAE Buffer|TAE Buffer]]
<br>
<br>
-
[[Erickson:TB]]
+
[[Erickson:TB|TB]]
==Antibiotics==
==Antibiotics==
[[Erickson:Kanamycin|Kanamycin]]
[[Erickson:Kanamycin|Kanamycin]]

Revision as of 18:24, 14 May 2009

Contents

PCR

Colony PCR
Real-Time PCR

RNA

RNA Isolation Protocol (TRIzol)

RNA Isolation Protocol (Mini Shredder)

Fleas

Flea Bedding

Media

LB mixture (includes plates mix)
LB Buffer
TAE Buffer
TB

Antibiotics

Kanamycin
Carbenacillin
Tetracycline
Irgasan
Chloramphenicol

Transposon Mutagenesis

Tri-parental Mating

Electroporation

Bi-parental Mating

Mutant Generation

Image:Making a mutant.pptx

Competent Cell Generation

Competent Cells

Personal tools