Escherichia coli
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Revision as of 13:39, 20 April 2007
Contents |
General pages
- Bacterial artificial chromosomes
- ColE1
- E. coli genotypes
- E. coli restriction-modification system
- Endy:High-efficiency electroporation
- Escherichia coli/Codon usage
- Escherichia coli/Knockouts
- Escherichia coli/Methylation
- Escherichia coli/Nomenclature & Abbreviations
- Escherichia coli/Vectors
- F plasmids
- Klapperich Lab:Streaking bacteria on Agar plate
- Moore:Working with coli
- P15A
- PSC101
- Recombineering
- Standard E. coli Strain for BioBricks
- Titratable control of pBAD and lac promoters in individual E. coli cells
- Transposable elements
Protocols
- Bacterial cell culture
- Bacterial transformation
- Belcher/Knight: Electrocompetent Cells
- Beta-Galactosidase Assay (A better Miller)
- Cell and tissue lysis hub
- Cell counting/plating
- Cell cycle analysis
- ChIP-Chip E. coli
- Colony PCR
- Duffy:LIC Cloning
- Electrocompetent cells
- Electroporation
- Endy:Colony PCR
- Endy:E. coli Western Blot
- Endy:STN Chemostat Protocol
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- Erman's Lab:DNA Miniprep with Alkaline Lysis
- Huang: Agarose Slides for Imaging
- In-fusion biobrick assembly
- Knight:Beta-galactosidase assay
- Knight:Beta-galactosidase assay/96 well format
- Knight:Beta-galactosidase assay/Fluorescence
- Knight:Colony PCR
- Knight:DNA Spots
- Knight:Electroporation
- Knight:Miniprep low copy plasmids
- Knight:Preparing electrocompetent cells
- Knight:Preparing stabs
- Lory:Quick transformant screen
- Making a long term stock of bacteria
- Maxiprep of plasmid DNA from E. coli
- McClean:Competent Cells
- Miniprep
- Miniprep/GET buffer
- Miniprep/Kit-free high-throughput protocol
- Miniprep/Qiagen kit
- Miniprep/TENS miniprep
- One step 'miniprep' method for the isolation of plasmid DNA
- P1vir phage transduction
- Preparing chemically competent cells
- Preparing chemically competent cells (Inoue)
- Recombineering/Lambda red-mediated gene replacement
- Richard Lab:Electroporation of E. coli
- Richard Lab:Preparing electrocompetent cells
- Sauer:Lysing E. coli with Lysozymes
- Seth:Quick Transformant Screen
- TOP10 chemically competent cells
- Transforming chemically competent cells
- Transforming chemically competent cells (Inoue)
Materials
- Cell and tissue lysis hub
- Endy:M9 media/supplemented
- Escherichia coli RNA polymerase
- Knight:M9 supplemented media
- M9 medium
- M9 medium/minimal
- M9 medium/supplemented
- M9 medium/with agar
- Neidhardt EZ Rich Defined
- PTXB1
- Streptomyces:Other Bits/Antibiotic and Resistance Marker Information
- TSS
Notes
- In an Escherichia coli cell, 1 molecule per cell is approximately equal to 1nM.
Resources
- Escherichia coli statistics by Institute for Biomolecular Design
- Several tables describing statistical data on E. coli compiled from several sources.
- Physiology of the Bacterial Cell by Neidhardt, Ingraham and Schaechter
- Reference text on bacterial cell physiology.
- EcoSal by ASM Press
- An incomplete web version of the publication Escherichia coli and Salmonella: Cellular and Molecular Biology. [Subscription required]
- EcoGene
- Database of all E. coli genes and sequences
- Ecocyc
- Comprehensive biochemical pathway and gene function site for E. coli
