Escherichia coli/Knockouts: Difference between revisions
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The [http://ecoli.aist-nara.ac.jp/gb6/Resources/deletion/deletion.html Keio collection] is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the [http://ecoli.aist-nara.ac.jp/ Functional Genomic Analysis of E. coli in Japan] project <cite>Baba-MSB-2006</cite>. | The [http://ecoli.aist-nara.ac.jp/gb6/Resources/deletion/deletion.html Keio collection] is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the [http://ecoli.aist-nara.ac.jp/ Functional Genomic Analysis of E. coli in Japan] project <cite>Baba-MSB-2006</cite>. | ||
These strains are currently available from a number of sources: | |||
[http:// | The [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do National Institute of Genetics] in Japan, or the [http://cgsc.biology.yale.edu/KeioList.php E. coli Genetic Stock Center (CGSC)]. | ||
Alternatively, you can obtain the relevant plasmids for doing the knockouts yourself from the [http://cgsc.biology.yale.edu/ | The entire collection as a set can also be obtained from [http://www.openbiosystems.com/GeneExpression/Non%2DMammalian/Bacteria/Keio/ Open Biosystems] | ||
Alternatively, you can obtain the relevant plasmids and strains for doing the knockouts yourself from the [http://cgsc.biology.yale.edu/GDK.php CGSC] (the "Wanner gene disruption kit")<cite>Datsenko-PNAS-2000</cite>. | |||
==References== | ==References== | ||
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==Source== | ==Source== | ||
*[http://lists.syntheticbiology.org/pipermail/discuss/2006q2/000132.html Email correspondence] between John Cumbers and Wilson Wong. | *[http://lists.syntheticbiology.org/pipermail/discuss/2006q2/000132.html Email correspondence] between John Cumbers and Wilson Wong. | ||
[[Category:Escherichia coli]] | |||
Latest revision as of 14:07, 14 March 2010
The Keio collection is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the Functional Genomic Analysis of E. coli in Japan project [1].
These strains are currently available from a number of sources:
The National Institute of Genetics in Japan, or the E. coli Genetic Stock Center (CGSC).
The entire collection as a set can also be obtained from Open Biosystems
Alternatively, you can obtain the relevant plasmids and strains for doing the knockouts yourself from the CGSC (the "Wanner gene disruption kit")[2].
References
- Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, and Mori H. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol. 2006;2:2006.0008. DOI:10.1038/msb4100050 |
- Datsenko KA and Wanner BL. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. DOI:10.1073/pnas.120163297 |
Source
- Email correspondence between John Cumbers and Wilson Wong.
