Escherichia coli/Knockouts

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Current revision (17:07, 14 March 2010) (view source)
(updated to show additional sources for Keio strains)
 
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The [http://ecoli.aist-nara.ac.jp/gb6/Resources/deletion/deletion.html Keio collection] is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the [http://ecoli.aist-nara.ac.jp/ Functional Genomic Analysis of E. coli in Japan] project <cite>Baba-MSB-2006</cite>.
The [http://ecoli.aist-nara.ac.jp/gb6/Resources/deletion/deletion.html Keio collection] is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the [http://ecoli.aist-nara.ac.jp/ Functional Genomic Analysis of E. coli in Japan] project <cite>Baba-MSB-2006</cite>.
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You can request strains from them.
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These strains are currently available from a number of sources:
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[http://ecoli.aist-nara.ac.jp/Resource/ResourceManage.jsp Request form] - note that they are quoting a shipping delay of 2 months due to so many requests.
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The [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do National Institute of Genetics] in Japan, or the [http://cgsc.biology.yale.edu/KeioList.php E. coli Genetic Stock Center (CGSC)].
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Alternatively, you can obtain the relevant plasmids for doing the knockouts yourself from the [http://cgsc.biology.yale.edu/cgsc.html CGSC] (pKD13 and pKD46 are needed most likely)<cite>Datsenko-PNAS-2000</cite>.
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The entire collection as a set can also be obtained from [http://www.openbiosystems.com/GeneExpression/Non%2DMammalian/Bacteria/Keio/ Open Biosystems]
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Alternatively, you can obtain the relevant plasmids and strains for doing the knockouts yourself from the [http://cgsc.biology.yale.edu/GDK.php CGSC] (the "Wanner gene disruption kit")<cite>Datsenko-PNAS-2000</cite>.
==References==
==References==

Current revision

The Keio collection is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the Functional Genomic Analysis of E. coli in Japan project [1].

These strains are currently available from a number of sources:

The National Institute of Genetics in Japan, or the E. coli Genetic Stock Center (CGSC).

The entire collection as a set can also be obtained from Open Biosystems

Alternatively, you can obtain the relevant plasmids and strains for doing the knockouts yourself from the CGSC (the "Wanner gene disruption kit")[2].

References

  1. Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, and Mori H. . pmid:16738554. PubMed HubMed [Baba-MSB-2006]
  2. Datsenko KA and Wanner BL. . pmid:10829079. PubMed HubMed [Datsenko-PNAS-2000]
All Medline abstracts: PubMed HubMed

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