Escherichia coli/Knockouts - Revision history

John Wertz: updated to show additional sources for Keio strains

2010-03-14T21:07:34Z

updated to show additional sources for Keio strains

←Older revision		Revision as of 21:07, 14 March 2010
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	The [http://ecoli.aist-nara.ac.jp/gb6/Resources/deletion/deletion.html Keio collection] is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the [http://ecoli.aist-nara.ac.jp/ Functional Genomic Analysis of E. coli in Japan] project <cite>Baba-MSB-2006</cite>.		The [http://ecoli.aist-nara.ac.jp/gb6/Resources/deletion/deletion.html Keio collection] is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the [http://ecoli.aist-nara.ac.jp/ Functional Genomic Analysis of E. coli in Japan] project <cite>Baba-MSB-2006</cite>.

-	~~You can request~~ strains from ~~them.~~	+	These strains are currently available from a number of sources:

-	[http://~~ecoli~~.~~aist-nara~~.ac.jp/~~Resource~~/~~ResourceManage~~.~~jsp Request form] - note that they are quoting a shipping delay~~ of ~~2 months due to so many requests~~.	+	The [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do National Institute of Genetics] in Japan, or the [http://cgsc.biology.yale.edu/KeioList.php E. coli Genetic Stock Center (CGSC)].

-	Alternatively, you can obtain the relevant plasmids for doing the knockouts yourself from the [http://cgsc.biology.yale.edu/~~cgsc~~.~~html~~ CGSC] (~~pKD13 and pKD46 are needed most likely~~)<cite>Datsenko-PNAS-2000</cite>.	+	The entire collection as a set can also be obtained from [http://www.openbiosystems.com/GeneExpression/Non%2DMammalian/Bacteria/Keio/ Open Biosystems]
		+
		+	Alternatively, you can obtain the relevant plasmids and strains for doing the knockouts yourself from the [http://cgsc.biology.yale.edu/GDK.php CGSC] (the "Wanner gene disruption kit")<cite>Datsenko-PNAS-2000</cite>.

	==References==		==References==

Reshma P. Shetty at 22:58, 5 December 2006

2006-12-05T22:58:05Z

←Older revision		Revision as of 22:58, 5 December 2006
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	==Source==		==Source==
	*[http://lists.syntheticbiology.org/pipermail/discuss/2006q2/000132.html Email correspondence] between John Cumbers and Wilson Wong.		*[http://lists.syntheticbiology.org/pipermail/discuss/2006q2/000132.html Email correspondence] between John Cumbers and Wilson Wong.
		+
		+	[[Category:Escherichia coli]]

Reshma P. Shetty at 16:34, 27 June 2006

2006-06-27T16:34:38Z

New page

The [http://ecoli.aist-nara.ac.jp/gb6/Resources/deletion/deletion.html Keio collection] is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the [http://ecoli.aist-nara.ac.jp/ Functional Genomic Analysis of E. coli in Japan] project <cite>Baba-MSB-2006</cite>.

You can request strains from them.

[http://ecoli.aist-nara.ac.jp/Resource/ResourceManage.jsp Request form] - note that they are quoting a shipping delay of 2 months due to so many requests.

Alternatively, you can obtain the relevant plasmids for doing the knockouts yourself from the [http://cgsc.biology.yale.edu/cgsc.html CGSC] (pKD13 and pKD46 are needed most likely)<cite>Datsenko-PNAS-2000</cite>.

==References==
<biblio>
#Baba-MSB-2006 pmid=16738554
#Datsenko-PNAS-2000 pmid=10829079
</biblio>

==Source==
*[http://lists.syntheticbiology.org/pipermail/discuss/2006q2/000132.html Email correspondence] between John Cumbers and Wilson Wong.