Escherichia coli/Knockouts

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The Keio collection is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the Functional Genomic Analysis of E. coli in Japan project [1].

You can request strains from them.

Request form - note that they are quoting a shipping delay of 2 months due to so many requests.

Alternatively, you can obtain the relevant plasmids for doing the knockouts yourself from the CGSC (pKD13 and pKD46 are needed most likely)[2].


Error fetching PMID 16738554:
Error fetching PMID 10829079:
  1. Error fetching PMID 16738554: [Baba-MSB-2006]
  2. Error fetching PMID 10829079: [Datsenko-PNAS-2000]
All Medline abstracts: PubMed HubMed


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