Escherichia coli/Knockouts

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The Keio collection is a collection of systematic single-gene knock-out mutants of E. coli K-12 done by the Functional Genomic Analysis of E. coli in Japan project [1].

You can request strains from them.

Request form - note that they are quoting a shipping delay of 2 months due to so many requests.

Alternatively, you can obtain the relevant plasmids for doing the knockouts yourself from the CGSC (pKD13 and pKD46 are needed most likely)[2].

References

1. Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, and Mori H. . pmid:16738554. PubMed HubMed [Baba-MSB-2006]
2. Datsenko KA and Wanner BL. . pmid:10829079. PubMed HubMed [Datsenko-PNAS-2000]

Source

• Email correspondence between John Cumbers and Wilson Wong.