Escherichia coli/Nomenclature & Abbreviations

A listed gene name means that gene carries a loss of function mutation, a Δ preceding a gene name means the gene is deleted. If a gene is not listed, it is not known to be mutated. Prophages present in wt K-12 strains (F, λ, e14, rac) are listed only if absent. E. coli B strains are naturally lon- and dcm-.

• F^- = Does not carry the F plasmid
• F⁺ = Carries the F plasmid. The cell is able to mate with F^- through conjugation.
• F'[ ] = Carries an F plasmid that has host chromosomal genes on it from a previous recombination event. This cell can also mate with F^- through conjugation. Chromosomal genes carried in the F plasmid are listed in brackets.
• r_B/K^+/- = The (B/K) defines the strain lineage. The +/- indicates whether the strain has or hasn't got the restriction system.
• m_B/K^+/- = The (B/K) defines the strain lineage. The +/- indicates whether the strain has or hasn't got the modification (methylation) system.
• hsdS = Both restriction and methylation of certain sequences is deleted from the strain. If you transform DNA from such a strain into a wild type strain, it will be degraded.
• hsdR = For efficient transformation of cloned unmethylated DNA from PCR amplifications
• INV( ) = chromosomal inversion between locations indicated
• ahpC = mutation to alkyl hydroperoxide reductase conferring disulfide reductase activity
• ara-14 = cannot metabolize arabinose
• araD = mutation in L-ribulose-phosphate 4-epimerase blocks arabinose metabolism
• cycA = mutation in alanine transporter; cannot use alanine as a carbon source
• dapD = mutation in succinyl diaminopimelate aminotransferase leads to succinate or (lysine + methionine) requirement
• Δ( ) = chromosomal deletion of genes between the listed genes (may include unlisted genes!)
• dam = adenine methylation at GATC sequences abolished; high recombination efficiency; DNA repair turned on
• dcm = cytosine methylation at second C of CCWGG sites abolished
• deoR = regulatory gene that allows constitutive expression of deoxyribose synthesis genes; permits uptake of large plasmids. See Hanahan D, US Patent 4,851,348.
• dnaJ = one of the chaparonins inactivated; stabilizes some mutant proteins
• dut1 = dUTPase activity abolished, leading to increased dUTP concentrations, allowing uracil instead of thymine incorporation in DNA. Stable U incorporation requires ung gene mutation as well.
• endA1 = For cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
• (e14) = excisable prophage like element containing mcrA gene; present in K-12 but missing in many other strains
• galE = mutant more resistant to phage P1 infection
• galK/U = cannot metabolize galactose
• gor = mutation in glutathione reductase; enhances disulphide bond formation
• glnV = suppression of amber (UAG) stop codons by insertion of glutamine; required for some phage growth
• gyrA96 = mutation in DNA gyrase; conveys nalidixic acid resistance
• gyrA462 = mutation in DNA gyrase; conveys resistance to ccdB colicin gene product
• hflA150 = protease mutation stabilizing phage cII protein; high frequency of lysogenization by λ
• Δ(lac)X74 = Deletion of the entire lac operon as well as some flanking DNA.
• lacI^q or lacI^Q = overproduction of the lac repressor protein; -35 site in promoter upstream of lacI is mutated from GCGCAA to GTGCAA
• lacI^Q1 = overproduction of the lac repressor protein; contains a 15 bp deletion to create optimal -35 site in promoter upstream of lacI
• lacY = deficient in lactose transport; deletion of lactose permease (M protein)
• lacZΔM15 = partial deletion of the lacZ gene that allows α complementation of the β-galactosidase gene; required for blue/white selection on XGal plates. Deletes the amino portion of lacZ (aa 11-41).
• leuB = requires leucine
• Δlon = deletion of the lon protease
• malA = cannot metabolize maltose
• mcrA = Mutation eliminating restriction of DNA methylated at the sequence C^mCGG (possibly ^mCG). Carried on the e14 prophage (q.v.)
• mcrB = Mutation eliminating restriction of DNA methylated at the sequence R^mC
• metB = requires methionine
• metC = requires methionine
• mrr = Mutation eliminating restriction of DNA methylated at the sequence C^mAG or G^mAC
• mtlA = cannot metabilize mannitol
• (Mu) = Mu prophage present. Muδ means the phage is defective.
• mutS - mutation inhibits DNA repair of mismatches in unmethylated newly synthesized strands
• nupG = same as deoR
• ompT = mutation in outer membrane protein protease VII, reducing proteolysis of expressed proteins
• (P1) = Cell carries a P1 prophage. Cells express the P1 restriction system.
• (P2) = Cell carries a P2 prophage. Allows selection against Red+ Gam+ λ
• (φ80) = Cell carries the lambdoid prophage φ80. A defective version of this phage carrying lacZM15 deletion (as well as lacIq, lacYA, and flanking sequences) is present in some strains. The φ80 attachment site is just adjacent to tonB.
• pLysS = contains pLysS plasmid carrying chloramphenicol resistance and phage T7 lysozyme, effective at attenuating activity of T7 RNA polymerase, for better inhibition of expression under non-induced conditions. The sequence can be found here.
• proA/B = requires proline
• recA1 = For reduced occurrence of unwanted recombination in cloned DNA; cells UV sensitive, deficient in DNA repair
• recA13 = as for recA1, but inserts less stable.
• recBCD = Exonuclease V; mutation in RecB or RecC reduces general recombination by a factor of 100; impaired DNA repair; UV sensitive, easier propagation of inverted repeats
• recJ Exonuclease involved in alternate recombination
• relA = relaxed phenotype; permits RNA synthesis in absence of protein synthesis
• rha = blocked rhamose metabolism
• rnc = encodes RnaseIII (rnc-14 is a common null mutant)
• rne = encodes RnaseE (rne-3071 is a common temperature sensitive mutant)
• rpsL = mutation in ribosomal protein S12 conveying streptomycin resistance; also called strA
• sbcBC = ExoI activity abolished; usually present in recBC strains; recombination proficient, stable inverted repeats
• srl = cannot metabolize sorbitol
• supE = glnV
• supF = tyrT
• thi = requires thiamine
• thyA = requires thymidine
• Tn10 = transposon normally carrying Tetracycline resistance
• Tn5 = transposon normally carrying Kanamycin resistance
• tonA = Mutation in outer membrane protein conveying resistance to phage T1 and phage T5
• traD = Mutation eliminating transfer factor; prevents transfer of F plasmid
• trxB = mutation in thioredoxin reductase; enhances disulphide bond formation in the cytoplasm
• tsx = outer membrane protein mutation conveying resistance to phage T6 and colicin K
• tyrT = suppression of amber (UAG) stop codons by insertion of tyrosine; needed for some phage infection such as λgt11.
• ung1 = allows uracil to exist in plasmid DNA
• xyl-5 = blocked xylose metabolism

• Sm^R = Streptomycin resistance