Escherichia coli RNA polymerase
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*0.5 mM NTPs | *0.5 mM NTPs | ||
*1μg T7 D111 DNA as template | *1μg T7 D111 DNA as template | ||
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Revision as of 09:30, 19 March 2007
Epicentre is the only commercial source for Escherichia coli RNA polymerase.
Contents |
Materials
Reaction conditions
For core enzyme
from Epicentre
For sigma saturated holoenzyme
from Epicentre
Functional test
50 μL reaction
- 1X E. coli RNA polymerase transcription buffer
- 0.04 M Tris-HCl (pH 7.5)
- 0.15 M KCl
- 10 mM MgCl2
- 0.01% Triton® X-100
- 2mM DTT
- 0.25mM NTPs
- 1μg T7 D111 DNA as template
Assay test
50 μL reaction
- 1X E. coli RNA polymerase transcription buffer
- 0.04 M Tris-HCl (pH 7.5)
- 0.15 M KCl
- 10 mM MgCl2
- 0.01% Triton® X-100
- 10 mM DTT
- 0.5 mM NTPs
- 1μg T7 D111 DNA as template
