Escherichia coli RNA polymerase: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 33: | Line 33: | ||
*1μg T7 D111 DNA as template | *1μg T7 D111 DNA as template | ||
[[Category:Escherichia coli]] | [[Category:Escherichia coli]] [[Category:Material]] [[Category:Enzyme]] [[Category:Polymerase]] [[Category:RNA]] | ||
Latest revision as of 07:22, 24 May 2007
Epicentre is the only commercial source for Escherichia coli RNA polymerase.
Materials
Reaction conditions
For core enzyme
from Epicentre
For sigma saturated holoenzyme
from Epicentre
Functional test
50 μL reaction
- 1X E. coli RNA polymerase transcription buffer
- 0.04 M Tris-HCl (pH 7.5)
- 0.15 M KCl
- 10 mM MgCl2
- 0.01% Triton® X-100
- 2mM DTT
- 0.25mM NTPs
- 1μg T7 D111 DNA as template
Assay test
50 μL reaction
- 1X E. coli RNA polymerase transcription buffer
- 0.04 M Tris-HCl (pH 7.5)
- 0.15 M KCl
- 10 mM MgCl2
- 0.01% Triton® X-100
- 10 mM DTT
- 0.5 mM NTPs
- 1μg T7 D111 DNA as template
