Etchevers:Notebook/Genomics of hNCC/2008/12/10: Difference between revisions
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When I've made the table, will put up on GoogleDocs. | When I've made the table, will put up on GoogleDocs. | ||
* In [http://www.nature.com/emboj/journal/v16/n11/full/7590280a.html this paper from EMBO J], where Mycn was overexpressed under the TH promoter in mice which subsequently developed NB's, an ensuing effect they noted by CGH array was a gain or loss of chromosomal material: | |||
''"Thirteen tumors (all from hemizygous mice) showed gains and losses of chromosomal regions (Figure 8, Table II). In mice hemizygous for the MYCN transgene, chromosomal regions were most commonly gained on chromosomes 11 (5/16) and 17 (6/16). These regions are syntenic with human chromosomes 6 and '''17''' respectively (Copeland et al., 1993), both of which commonly are gained in human neuroblastoma (Plantaz et al., 1997). Chromosomal loss was most often detected on chromosomes 5 (4/16), 9 (3/16), 16 (4/16) and X (4/16). These chromosomes are syntenic with human chromosomes 4, 11, 3 and X respectively (Copeland et al., 1993), all of which commonly are lost in human neuroblastoma (Plantaz et al., 1997)."'' | |||
* If this were to be done today, perhaps targeting with Wnt1 promoter in a Cre-Lox format would be more appropriate - as TH is itself in one of the amplified intervals (chr11:2043796-2201921). | |||
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Revision as of 03:39, 10 December 2008
Genomics of human neural crest cells | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
R.I.P. and NBFinally threw away the last remnants of the cells that had been in Rich and Neural medium. There were still cells left in the "neural" culture but they were pretty much pericyte-like and those can hang on for months. The medium had evaporated, meanwhile (after 10 days!). When I was last in Paris I had established 2x C13 new hNCC cultures that Sophie says are growing well.
Looking at neuroblastoma data. In particular, cf. the paper by Chen et al. published at the same time as Janoueix-Lerosey et al. on the CNVs that were identified in NB cell lines.
When I've made the table, will put up on GoogleDocs.
"Thirteen tumors (all from hemizygous mice) showed gains and losses of chromosomal regions (Figure 8, Table II). In mice hemizygous for the MYCN transgene, chromosomal regions were most commonly gained on chromosomes 11 (5/16) and 17 (6/16). These regions are syntenic with human chromosomes 6 and 17 respectively (Copeland et al., 1993), both of which commonly are gained in human neuroblastoma (Plantaz et al., 1997). Chromosomal loss was most often detected on chromosomes 5 (4/16), 9 (3/16), 16 (4/16) and X (4/16). These chromosomes are syntenic with human chromosomes 4, 11, 3 and X respectively (Copeland et al., 1993), all of which commonly are lost in human neuroblastoma (Plantaz et al., 1997)."
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