Etchevers:Notebook/Genomics of hNCC/2009/03/23: Difference between revisions
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== | ==Survival and plans for the week== | ||
All cells looked good, even those plated on non-collagen dishes (but these like all the others look pretty actin-fiber-filled). Some cell death but not over-much. | |||
Should be working in quarantine now because re-thawed cells, until the mycoplasma test is finished. | |||
Rat tail collagen from BD Biosciences, ref 354236 lot 32334. This is at 3.92 mg/mL. Should dilute to 50 μg/mL. This is a factor of dilution = 78.4. Dilute in PBS, though BD suggests in 0.02N acetic acid? (Millipore in 0.01N and I think last time, I used PBS. No wonder it agglomerated.) | |||
*'''[[User:Etchevers|Heather]] 04:14, 23 March 2009 (EDT)''': | |||
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Revision as of 01:14, 23 March 2009
 Genomics of human neural crest cells
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Survival and plans for the weekAll cells looked good, even those plated on non-collagen dishes (but these like all the others look pretty actin-fiber-filled). Some cell death but not over-much. Should be working in quarantine now because re-thawed cells, until the mycoplasma test is finished. Rat tail collagen from BD Biosciences, ref 354236 lot 32334. This is at 3.92 mg/mL. Should dilute to 50 μg/mL. This is a factor of dilution = 78.4. Dilute in PBS, though BD suggests in 0.02N acetic acid? (Millipore in 0.01N and I think last time, I used PBS. No wonder it agglomerated.)
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