Etchevers:Notebook/STRA6 in eye development/2009/05/11: Difference between revisions
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(Autocreate 2009/05/11 Entry for Etchevers:Notebook/STRA6_in_eye_development) |
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== | ==Bacteria and HEK 293 cells and HIS head genes== | ||
Adeline has used some of the competent bacteria. There is remaining - one x 400μL aliquot at -70°C in box I, spot A3. Would like to get the Google Docs spreadsheet up and running. Stephane has transfected into 2x 50 μL from the other remaining large aliquot and will replace 6x aliquots into my yellow box. Adeline will use four of these. | |||
DH5a competent NEB #C2988J to replace when necessary. | |||
Diluted pCAGGS-GFP that Christelle sent me, 1400ng/μL, to 28 ng/μL (1/50) for Stephane to use 5 μL and transfect my bacteria for control transfections. Need to find out if there is a transilluminator that makes GFP glow. | |||
*'''[[User:Etchevers|Heather]] 08:21, 11 May 2009 (EDT)''': | |||
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Revision as of 05:21, 11 May 2009
 Genetics of human eye development
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Bacteria and HEK 293 cells and HIS head genesAdeline has used some of the competent bacteria. There is remaining - one x 400μL aliquot at -70°C in box I, spot A3. Would like to get the Google Docs spreadsheet up and running. Stephane has transfected into 2x 50 μL from the other remaining large aliquot and will replace 6x aliquots into my yellow box. Adeline will use four of these. DH5a competent NEB #C2988J to replace when necessary. Diluted pCAGGS-GFP that Christelle sent me, 1400ng/μL, to 28 ng/μL (1/50) for Stephane to use 5 μL and transfect my bacteria for control transfections. Need to find out if there is a transilluminator that makes GFP glow.
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