Etchevers:Notebook/STRA6 in eye development/2009/05/18: Difference between revisions
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But with the clumps, today, there were approximately 255 x 10<sup>4</sup> cells/mL - seeded 50K, 100K and 150K cells per well of 24 well plate. This corresponded respectively to 0.48, 1.92 and 1.44 mL cells in 23.5 mL, 46 mL or 22.56 mL medium (two dishes of 100K cells/dish, one on an expired Corning plastic. This grew just fine.) | But with the clumps, today, there were approximately 255 x 10<sup>4</sup> cells/mL - seeded 50K, 100K and 150K cells per well of 24 well plate. This corresponded respectively to 0.48, 1.92 and 1.44 mL cells in 23.5 mL, 46 mL or 22.56 mL medium (two dishes of 100K cells/dish, one on an expired Corning plastic. This grew just fine.) | ||
Also froze 5x ampoules in liquid nitrogen with 2.5 million cells per ampoule (spaces 3-8 in 5th row, yellow tier). | |||
*'''[[User:Etchevers|Heather]] 11:47, 25 May 2009 (EDT)''': | *'''[[User:Etchevers|Heather]] 11:47, 25 May 2009 (EDT)''': | ||
Revision as of 08:57, 25 May 2009
 Genetics of human eye development
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Seeding HEK 293T cells for extractionT75 flask had 23 million cells as per counting. I taught Adeline how to count on clumpy cells that had just been detached by PBS - to avoid in the future. But with the clumps, today, there were approximately 255 x 104 cells/mL - seeded 50K, 100K and 150K cells per well of 24 well plate. This corresponded respectively to 0.48, 1.92 and 1.44 mL cells in 23.5 mL, 46 mL or 22.56 mL medium (two dishes of 100K cells/dish, one on an expired Corning plastic. This grew just fine.) Also froze 5x ampoules in liquid nitrogen with 2.5 million cells per ampoule (spaces 3-8 in 5th row, yellow tier).
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