Ethanol precipitation of nucleic acids

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
This is now empty. I will post stuff later
+
To your sample add
 +
    3 volumes of 100% Ethanol
 +
    1/10 volume of 3M sodium acetate
 +
 
 +
Mix and freeze overnight in -20. This step some say is unnecessary but others swear by. If you are in a rush you can also put it in the -80 for a few hours.
 +
 
 +
Spin at 13000 RPM, 4 degrees for 30 minutes on your benchtop centrifuge. Make sure to mark the outermost edge of the tube so you can find the pellet easily. It is clear and usually looks like a little smudge on the tube.
 +
 
 +
Decant the supernatant.
 +
 
 +
Add your desired quantity of water. Vortex and spin down to resuspend.

Revision as of 17:19, 13 September 2005

To your sample add

    3 volumes of 100% Ethanol
    1/10 volume of 3M sodium acetate

Mix and freeze overnight in -20. This step some say is unnecessary but others swear by. If you are in a rush you can also put it in the -80 for a few hours.

Spin at 13000 RPM, 4 degrees for 30 minutes on your benchtop centrifuge. Make sure to mark the outermost edge of the tube so you can find the pellet easily. It is clear and usually looks like a little smudge on the tube.

Decant the supernatant.

Add your desired quantity of water. Vortex and spin down to resuspend.

Personal tools