Exp1: test for flipping and cross-talk: Difference between revisions
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(New page: The first experiment is design to test if integrases and excisionases pairs can flip a target sequence in both directions in a controlled manner. Then, we will test if these integrases are...) |
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Revision as of 20:06, 13 January 2009
The first experiment is design to test if integrases and excisionases pairs can flip a target sequence in both directions in a controlled manner. Then, we will test if these integrases are specific by putting them in presence of non-target sites. If this first set of experiment is successfull, we will then perform a kinetic analysis of flipping, by varying time of induction and concentrations of recombinases.
In a first time we will characterize [Integrase/Excisionase pairs to test 3 pairs]
==Integrases/Excisionases pairs to test==3 pairs
