F plasmids: Difference between revisions
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==F plasmid derived replication origins== | ==F plasmid derived replication origins== | ||
There are at least 3 kinds of replication origins that are derived from the F factor of ''Escherichia coli''. | There are at least 3 kinds of replication origins that are derived from the [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=9507713 F factor] of ''Escherichia coli''. | ||
===mini-F=== | ===mini-F=== | ||
*includes oriS and repE | *includes oriS and repE | ||
*~1.6 kb | *~1.6 kb | ||
*used in pSCANS or [[Synthetic Biology:Vectors/Nomenclature|pSB2K3-P1010]] series plasmids | *used in pSCANS ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Nucleotide&cmd=Retrieve&list_uids=6689911&dopt=GenBank genbank] [http://genome.bnl.gov/Vectors/pscans.php vector info] [http://www.genome.bnl.gov/Protocols/pscans.shtml cookbook]) or [[Synthetic Biology:Vectors/Nomenclature|pSB2K3-P1010]] series plasmids | ||
*at copy number 4-6? | *at copy number 4-6? | ||
| Line 13: | Line 13: | ||
*includes oriS, repE and the par genes | *includes oriS, repE and the par genes | ||
*~4.6 kb | *~4.6 kb | ||
*used in pBeloBAC11 and other [[Bacterial artificial chromosomes|BACs]] | *used in [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=1817728 pBeloBAC11] and other [[Bacterial artificial chromosomes|BACs]] | ||
*at copy number 4-6 | *at copy number 4-6 | ||
| Line 45: | Line 45: | ||
#GeneticsGlossary [http://www.emunix.emich.edu/~rwinning/genetics/glossary.htm Biol301 at Eastern Michigan University] | #GeneticsGlossary [http://www.emunix.emich.edu/~rwinning/genetics/glossary.htm Biol301 at Eastern Michigan University] | ||
</biblio> | </biblio> | ||
[[Category:Escherichia coli]] | |||
Latest revision as of 10:31, 20 April 2007
in progress!
F plasmid derived replication origins
There are at least 3 kinds of replication origins that are derived from the F factor of Escherichia coli.
mini-F
- includes oriS and repE
- ~1.6 kb
- used in pSCANS (genbank vector info cookbook) or pSB2K3-P1010 series plasmids
- at copy number 4-6?
F plasmid derived origin used in most BACs
- includes oriS, repE and the par genes
- ~4.6 kb
- used in pBeloBAC11 and other BACs
- at copy number 4-6
"complete" F origin
- includes repC, oriV, ccd operon, repD, oriS, repE and the par genes
- ~9kb
- used by Kris Prather in her F plasmids that she built when in Jay Keasling's lab [1]
- at copy number 1-2
Nomenclature
- Episome: A circular piece of DNA that can replicate independently of the bacterial chromosome or integrate and replicate as part of the chromosome.
- F factor: An episome in bacterial cells that confers the ability to act as a genetic donor during conjugation.
- F+ cell: A bacterial cell possessing the fertility factor. Acts as a donor in conjugation.
- F- cell: A bacterial cell that does not contain a fertility factor. Acts as a recipient in conjugation.
- F' cell: A bacterial cell in which an integrated fertility factor spontaneously excises itself, taking part of the chromosome with it. The resulting episome therefore contains chromosomal material.
- Hfr cell: A strain of bacteria exhibiting a high frequency of recombination, due to a chromosomally-integrated fertility factor. This strain is able to transfer all or part of the chromosome to recipient strains.
From [2].
Mating types
- F+ mating with F- produces 2 F+
- Hfr mating with F- produces Hfr and F-
- F' mating with F- produces F' and F' merozygote
From [2].
